Isolation and partial purification of cell wall lipopolysaccharides of Pseudomonas Aeruginosa and using it as vaccine
Other Title(s)
فصل و تنقية جزئية للايبوبولي سكرايد من جدار بكتريا سيدوموناس ارجينوزا و استعماله كلقاح
Joint Authors
Mahdi, Sawsan Abd al-Husayn
Muhammad, Taghrid Ulum
Source
Ibn al-Haitham Journal for Pure and Applied Science
Issue
Vol. 30, Issue 1 (30 Apr. 2017), pp.88-95, 8 p.
Publisher
University of Baghdad College of Education for Pure Science / Ibn al-Haitham
Publication Date
2017-04-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
This study ,the samples were collected from "118 patients " suffering from burn wound contaminated with Pseudomonas aeruginosa and 100 health individuals (male and female ) as a control group ,the samples were wound swap and blood sample .
Chromatography technique was employed to extract and purify cell wall containing lipopolysaccharide by using P.
aeruginosa isolate ATCC 15692,the purification done by addition of ammonuium sulfate, sodium dodecyl sulfat (SDS) anddialysis, gel filtration chromatography by using sepharose-4B.
Immunogenicity of LPS component was determined by mice injection under the skin ,then Ab concentration against LPS component formed and determined by ELISA .
These findings indicated ability of LPS to make immune system stimulated to release specific Ab towards P.
aeruginosa isolate ATCC 15692 , therefore, this result may be a promising procedure to produce vaccine against P.
aeruginosa isolate ATCC 15692.
American Psychological Association (APA)
Muhammad, Taghrid Ulum& Mahdi, Sawsan Abd al-Husayn. 2017. Isolation and partial purification of cell wall lipopolysaccharides of Pseudomonas Aeruginosa and using it as vaccine. Ibn al-Haitham Journal for Pure and Applied Science،Vol. 30, no. 1, pp.88-95.
https://search.emarefa.net/detail/BIM-790552
Modern Language Association (MLA)
Muhammad, Taghrid Ulum& Mahdi, Sawsan Abd al-Husayn. Isolation and partial purification of cell wall lipopolysaccharides of Pseudomonas Aeruginosa and using it as vaccine. Ibn al-Haitham Journal for Pure and Applied Science Vol. 30, no. 1 (Apr. 2017), pp.88-95.
https://search.emarefa.net/detail/BIM-790552
American Medical Association (AMA)
Muhammad, Taghrid Ulum& Mahdi, Sawsan Abd al-Husayn. Isolation and partial purification of cell wall lipopolysaccharides of Pseudomonas Aeruginosa and using it as vaccine. Ibn al-Haitham Journal for Pure and Applied Science. 2017. Vol. 30, no. 1, pp.88-95.
https://search.emarefa.net/detail/BIM-790552
Data Type
Journal Articles
Language
English
Notes
Includes appendices : p. 92-94
Record ID
BIM-790552