Determination of toxoplasma gondii lineages of sheep in Wasit, Iraq
Other Title(s)
تحديد أنساب مقوسة كوندي في الأغنام في واسط، العراق
Author
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 30, Issue 2 (31 Dec. 2016), pp.23-26, 4 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
4
Main Subjects
Topics
Abstract EN
Toxoplasma gondii is an intracellular parasite that can cause significant morbidity in human beings and animals.
Up to our knowledge no data is known of genetic diversity of T.
gondii in sheep in Iraq.
This study aim to detect the strains (genotypes) of T.
gondii isolates from sheep in Wasit province, east of Iraq.
A total of 315 samples (blood 300 and placenta's tissue 15) were collected from aborted ewes, which initially had been examined serologically by LAT, then further tested by RT-PCR through B1 gene amplification to confirm the infection with T.
gondii.
After that, the positive DNA samples were assayed for genetic characterization depending upon nested PCR- RFLP of SAG2 gene.
Out of 315 examined samples, 10 were confirmed positive T.
gondii DNA.
The genotyping assay of them revealed that 60% (6/10), 30% (3/10) and 10% (1/10) of examined isolates represent the genotypes of II, III and I respectively.
The type II appeared as dominant in sheep in Wasit province, Iraq.
American Psychological Association (APA)
Ayiz, Numan Naji. 2016. Determination of toxoplasma gondii lineages of sheep in Wasit, Iraq. Iraqi Journal of Veterinary Sciences،Vol. 30, no. 2, pp.23-26.
https://search.emarefa.net/detail/BIM-791160
Modern Language Association (MLA)
Ayiz, Numan Naji. Determination of toxoplasma gondii lineages of sheep in Wasit, Iraq. Iraqi Journal of Veterinary Sciences Vol. 30, no. 2 (2016), pp.23-26.
https://search.emarefa.net/detail/BIM-791160
American Medical Association (AMA)
Ayiz, Numan Naji. Determination of toxoplasma gondii lineages of sheep in Wasit, Iraq. Iraqi Journal of Veterinary Sciences. 2016. Vol. 30, no. 2, pp.23-26.
https://search.emarefa.net/detail/BIM-791160
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 26
Record ID
BIM-791160