Histological and molecular study of spleen in Japanese quail under thermal condition

Abstract EN

he present study was conducted to determine the histological and Molecular changes in the spleen of adult male Japanese quail after being exposed to effect of thermal stress was recorded.

This study included two groups, each group consisting twelve male birds, the control group (A) was exposed to normal temperature for 45days, while the group (B) exposed to temperature (42OC) for 45 days.

The histological and molecular changes were studied during (15, 30 and 45) days of the experiment, histological changes in spleen were represented by necrosis, degeneration hemorrhage and shrinkage fibrosis of lymphocytic nodules, cytoplasmic vaculation, infiltration of lymphocytes, metamorphosis of lymphocyte, nuclei pyknotic, dilation in the central vein of lymphatic nodules, edema, dilation of sinusoids and congested blood vessels.

Molecular examination showed that the heat shock protein (hsp70) gen is present in temperature group that is also found in the control group.

This confirms that the hsp70 gen is present in birds at normal and abnormal conditions.