Determination of different species of animal from their meats by using PCR-RFLP technique of mitochondria gene COI

Abstract EN

This study was conducted at Dr.

Taleb A.

Jaayad of Molecular Genetics, Department of Animal Production, College of Agriculture, University of Basrah.

Samples of fresh and canned meat of cattle, buffalo, sheep, goats, chicken and turkey were collected randomly from different areas of Basrah province, as well as blood samples of camel.

The aim was to determine different animal species from their meat (except camel).

DNA was extracted from meat tissue (0.2 gm) and blood by using DNA kit (Invetrogen).

DNA purity was estimated by using wavelength (260-280), to be 1.8- 2.0 ng.

PCR was used to amplify mtco1 gene using a general primer and gave a band of 710 bp for all species used in this study.

Different species were determined by using Taq restricted enzyme.

Cattle, buffalo, chicken and turkey showed one band of 637 bp.

Taq enzyme has recognized sheep and goat, while sheep did not show any band to the fragment 710 bp.

However, goat showed a band at 650 bp.

Furthermore, camel produced two bands of 303 and 403 bp.