Molecular identification of peste des petits ruminants virus in wild goat and domestic small ruminants by real-time-PCR technique in Erbil-Iraq

Abstract EN

In July 2010 outbreak was occurred in wild goat in Barzan, Sherwin mizzen and Mergasur in Kurdistan Region- Iraq.

There were over 2700 deaths (both young and adult) during the period of July 2010 to October 2011.

Based on the clinical signs and post-mortem findings, the involvement of peste des petits ruminants virus (PPRV) was suspected.

This was confirmed by Real Time PCR technique using TaqMan®probes for the detection of Peste des petits ruminants.

The results of Real-Time PCR for the 9 sample taken from 9 Wild goat there are 6 sample positive and 3 sample negative and 76 sample from domestic ruminants (sheep and goat) 63 samples was negative for PPR.

This result confirms the diagnosis domestic ruminants in the region are routinely vaccinated with an attenuated vaccine based on the ‘Nigeria/75/1’ strain of PPRV.