Synthetic immunostimulatory glycans interference with host cell apoptosis upon of Toxoplasma gondii infection, in vitro
Other Title(s)
الكابوهيدرات المتعددة الاصطناعية تتداخل مع موت الخلية المبرمج للمضيف خلال تعرضها للإصابه بالمقوسات الغوندية، in verto
Author
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 31, Issue 1 (30 Jun. 2017), pp.43-49, 7 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2017-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Topics
Abstract EN
Toxoplasmosis is a protozoan infection of humans and animals caused by Toxoplasma gondii, and it’s continuous public health and food safety issue.
The tachyzoites (Tg) of T.
gondii are the most important stage, as they come in direct contact with immune cells such as a macrophage.
Tg can modulate and prevent apoptosis of immune cells while promoting survival of the pathogen.
Infections caused by Tg can be eradicated if immune cells could stimulate apoptosis and kill pathogens upon exposure.
Apoptosis is characterized by the release of mediators, namely Caspases (Cas).
New means are required for inducing apoptosis and enhance immunity in the infected host cell to control toxoplasmosis.
The present study investigated whether Synthetic Immuno-stimulatory Glycans (SIGs) influence Cas and Nitric oxide (NO) release and led to Tg damage.
Galβ1- 3Gal-PAA-fluor (SIG1), Fucα1-4GlcNAcβ-PAA-fluor (SIG2) and GlcNAcβ1-3GalNAcα-PAA-fluor (SIG3) constituted samples studied principally.
Murine macrophage had been exposed to the Tg then the SIGs effects on Cas and NO production were determined after 20 hours of pathogen phagocytosis.
Here we report that the SIGs had potent in vitro activity against T.
gondii; SIG2 was more effective than SIG1 and SIG3, representative by SIG2 treated infected macrophages can induced infected macrophages to release Cas1, 3, and 9.
Maximum production of NO by infected macrophages was noticed following the expoxure to all SIGs.
Therefore the present study provided the method for the selection of SIGs ligands bearing immunostimulatory factor and apoptotic stimuli properties.
American Psychological Association (APA)
Isa, Suzan Husayn. 2017. Synthetic immunostimulatory glycans interference with host cell apoptosis upon of Toxoplasma gondii infection, in vitro. Iraqi Journal of Veterinary Sciences،Vol. 31, no. 1, pp.43-49.
https://search.emarefa.net/detail/BIM-792292
Modern Language Association (MLA)
Isa, Suzan Husayn. Synthetic immunostimulatory glycans interference with host cell apoptosis upon of Toxoplasma gondii infection, in vitro. Iraqi Journal of Veterinary Sciences Vol. 31, no. 1 (2017), pp.43-49.
https://search.emarefa.net/detail/BIM-792292
American Medical Association (AMA)
Isa, Suzan Husayn. Synthetic immunostimulatory glycans interference with host cell apoptosis upon of Toxoplasma gondii infection, in vitro. Iraqi Journal of Veterinary Sciences. 2017. Vol. 31, no. 1, pp.43-49.
https://search.emarefa.net/detail/BIM-792292
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 48-49
Record ID
BIM-792292