Effects of some heavy metals on the activity and kinetics of peroxidase and polyphenol oxidase enzymes in petroselium cripsum, rosmarinus officinalis and eruca sativa

Other Title(s)

تأثير بعض المعادن الثقيلة على نشاط إنزيمات البيروكسيديز و البولي فينول أوكسيديز في البقدونس، إكليل الجبل و الجرجير

Dissertant

al-Butush, Manar Abd al-Salam

Thesis advisor

Atruz, Umar

University

Mutah University

Faculty

Faculty of Science

Department

Department of Biological Sciences

University Country

Jordan

Degree

Master

Degree Date

2013

English Abstract

The catalytic activities and the kinetic parameters of crude enzymes polyphenol oxidase (POX) and peroxidase (PPO) ezymes of crude extracts of Rosmarinus officinalis, Petroselium cripsum, and Eruca Sativa were invistigated in the presence and absence of various heavy metals.

The results showed that the crude enzyme (PPO) activity in the selected plants were increased in the presence of 400 μM of Cd2+, Co2+, Al3+, Fe3+ and Ni2+ as evidenced by the decrease in the Km and the increase in the Vmax values.

Meanwhile, the presence of some other heavy metals resulted in the activation of crude enzyme (PPO) extracts of Eruca Sativa and Rosmarinus officinalis such as Mo2+.

In contrast, other heavy metals such as Hg2+and Cr2+acted as a noncompetitive inhibitors in the crude enzyme (PPO) extracts of Petroselium cripsum, while Fe3+ and Pb2+ in crude enzyme (POX) of Rosmarinus officinalis and Eruca Sativa and Cr+2 and Co2+ in the Eruca Sativa extract (POX).

Moreover, Al3+ and Mo2+ acted as a noncompetitive inhibitors in the crude enzyme (POX) Rosmarinus officinalis extract.

Uncompetitive inhibition were observed in presence of Cu2+ and Pb2+ for crude enzyme ( PPO) extract of rosemary as evidenced by the decrease in the Km and the Vmax values.

In presence of Zn2+, the results revealed that the activity of crude enzyme ( PPO) of Petroselium cripsum were increased (activation).

Meanwhile, the presence of Zn2+ in the crude extract of Rosmarinus officinalis cause a noncompetitive inhibition and uncompetitive inhibition for PPO extracted in Eruca Sativa.

Regarding the effects of heavy metals on peroxidase activity, presence of Co2+, Fe3+, Hg2+ and Zn2+in the crude enzyme (POX) extract of Petroselium cripsum and Eruca Sativa have an activation effects .

In contrast, Cd2+, Co2+and Cu2+ have an uncompetitive inhibition for the selected plants.

Furthermore, Fe3+, Al3+, Pb2+ and Mo2+ were found to act as a noncompetitive inhibitors on the crude enzyme (POX) extract of Rosmarinus officinalis, while Cr+2, Co2+, Fe3+ and Pb2+ acted as a noncompetitive inhibitors on the crude enzyme (POX) extract Rosmarinus officinalis.

Presence of heavy metals altred these activities by acting as a uncompetitive or noncompetitive inhibitors or acting as activators depending on the type of heavy metals.

Main Subjects

Biology

No. of Pages

69

Table of Contents

• APA
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Language

English

Data Type

Arab Theses

Record ID

BIM-793332