Isolation and purification of staphylococcus aureus surface antigen b and using it for the diagnosis by elisa technique

Abstract EN

Staphylococcus aureus is the most common cause of staphylococcal infections.

It is a spherical bacterium, frequently lives on the skin or in the nose of a human.

Many tests were done for the diagnosis of Staphylococcus aureus isolated from blood culture, these include: culture, gram stain, catalase test, coagulase test and diagnosis by Vitek system.

The results appear that 90% were (H7) isolates while 10% were other isolate.

Chromatography technique was used for extraction, purification and isolation of the antigen (enterotoxin B) from the cell wall of Staphylococcus aureus.

ELISA technique was used for the detection of enterotoxin B.

This antigen (enterotoxin B) was employed for the production of diagnostic ELISA kit against staphylococci (particularly for Iraqi peoples).