Application of dynamic contrast-enhanced mri in the therapeutic evaluation of the chinese medicine kanglaite in treating colorectal cancer

Abstract EN

Background : Magnetic resonance imaging (MRI) can provide a reference for tumor treatment and its quantitative parameters can serve as imaging indicators that reflect tumor angiogenesis and vascularity.

Kanglaite (KLT) has therapeutic effects on cancers.

In this study, DCE-MRI was used to investigate its application in evaluating KLT anti-colorectal cancer.

Objectives : Evaluating the efficacy of Kanglaite (KLT) injection for treatment of colorectal cancer with dynamic contrast-enhanced MRI (DCE-MRI) parameters.

Methods : This study was an experimental study.

The 20 successfully modeled nude mice were randomly assigned to 2 groups: blank control group (n = 10) and the KLT injection group (n = 10).

The research protocol was approved by the ethics committee of the second affiliated hospital of Xinjiang medical university (protocol NO: 20140216-12) in 2015.

A subcutaneous xenograft colorectal tumor model was subjected to KLT treatment.

DCE-MRI obtain the parameters including Ktrans, Ve, Kep, Vp, immunohistochemical staining measure microvascular density (MVD), levels of vascular endothelial growth factor (VEGF), and proliferating cell nuclear antigen (PCNA).

Results : Compared to the blank control, the volume of tumor in the KLT group markedly reduced by 49%.

48 hours after, compared to the blank control, the Ktrans (0.0280.009 vs 0.0120.006), Ve (0.3120.089 vs 0.2870.037), and Kep values (0.3210.056 vs 0.577 0.033) decreased in KLT group (P < 0.05).

In contrast, the Vp value (0.094 0.037 vs 0.043 0.017) was significantly elevated in the KLT group (P < 0.05).

There is a correlation between Ktrans, Kep and VEGF score, MVD count, and PCNA score.

Conclusions : Theparameters of DCE-MRImaybeused asimagingbiomarkers for assessing the status of tumor-bearing vasculatures and provide a basis for evaluating the efficacy of anti-tumor drugs.