تقييم السائل المنوي المجمد في أوساط مختلفة عند كباش العواس باستخدام جهاز ال CASA

Abstract EN

The aim of this study was to determine the efficiency of local extenders in cryopreservation of semen Awassi rams.

The semen was collected from three rams by an artificial vagina method twice/ per day per week for 3 months.

This semen was diluted with three extenders (Sodium Citrate with Egg Yolk, EYC and Skimmed Milk, SM) and Andromed® as a control diluent.

The straws were filled by semen diluted and frozen automatically; the frozen straws were examined under CASA system.

The results showed that there was significant difference (P<0.001) in the rates of motility (MOT) and progressive (PROG), The rates were 63.42±1.13, 50.93±1.64, 31.76± 1.99, and 33.25± 2.63, 21.19±1.89% in the Andromed®, EYC and SM, respectively, and LIN was 45, 49, 58% in the Andromed®, EYC and SM respectively.

Distance Average Path (DAP) were 21.3±0.53 , 19.74±0.70, and 16.06±0.61 in the Andromed®, EYC and SM respectively, and Distance Straight Lin (DSL) were 15.61±0.32, 15.39±0.50, and 12.95±0.43 respectively, Distance Curved Lin (DCL) were 34.73±0.99, 31.32±1.29, and 22.88±1.23 respectively.

Also Velocity Average Path (VAP) were 52.41±1.33, 46.97±1.70, and 40.56±1.59 in the Andromed®, EYC and SM respectively, and Straight Lin Velocity (VSL) were 38.5±0.81, 36.7±1.20, and 32.9±1.16 respectively, also Curved Lin Velocity (VCL) were 84.70±2.46, 74.13±3.1, and 57.29±3.00 respectively.

Lateral Head Displacement (ALH) were 6.18±0.13, 5.64±0.16, and 4.80±0.21 in the Andromed®, EYC and SM, respectively.

It was concluded that EYC can be used as local extender to dilute the frozen semen of Awassi rams.