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Qualitative and quantitative estimation of keratin in some : Sudanese camel urine samples
Author
Source
Sudan Medical Laboratory Journal
Issue
Vol. 3, Issue 1 (30 Jun. 2016), pp.25-29, 5 p.
Publisher
Omdurman Islamic University Faculty of Medical Laboratory
Publication Date
2016-06-30
Country of Publication
Sudan
No. of Pages
5
Main Subjects
Abstract EN
Five samples of Sudanese camel urines were analyzed separately.
Protein of camel urine was hydrolyzed using HCl to extract amino acids.
When lead acetate was added, lead sulphide precipitate was formed(1).
This proved the presence of amino acids containing sulphur (cysteine and methionine).
The ninhydrin test showed the presence of α amino acids.
Alkaline Copper sulphate showed the presence of proteins, (Buirt test).
The absorbance peak of protein in the UV appeared at 350 nm indicating the presence of Keratin.
The presence of keratin was confirmed by TLC technique, where the standard camel urine samples had the same Rf value.
The average concentration of Keratin in camel urine was 0.037 M (5500ppm), which indicted that camel urine contained a high concentration of keratin.
American Psychological Association (APA)
Musa, Umar. 2016. Qualitative and quantitative estimation of keratin in some : Sudanese camel urine samples. Sudan Medical Laboratory Journal،Vol. 3, no. 1, pp.25-29.
https://search.emarefa.net/detail/BIM-796456
Modern Language Association (MLA)
Musa, Umar. Qualitative and quantitative estimation of keratin in some : Sudanese camel urine samples. Sudan Medical Laboratory Journal Vol. 3, no. 1 (2016), pp.25-29.
https://search.emarefa.net/detail/BIM-796456
American Medical Association (AMA)
Musa, Umar. Qualitative and quantitative estimation of keratin in some : Sudanese camel urine samples. Sudan Medical Laboratory Journal. 2016. Vol. 3, no. 1, pp.25-29.
https://search.emarefa.net/detail/BIM-796456
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 29
Record ID
BIM-796456