Molecular detection of suspected leishmania isolates using polymerase chain reaction
Other Title(s)
التحري الجزيئي لعزلتين مشكوك بهما لطفيلي اللشمانيا باستخدام تفاعل البلمرة المتسلسل
Joint Authors
Bayram, Duha Muhanad
al-Samirrai, Haydar Zuhayr Ali
Source
Issue
Vol. 58, Issue 4B (31 Dec. 2017), pp.2076-2082, 7 p.
Publisher
University of Baghdad College of Science
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
Leishmaniasis is a widespread parasitic disease that occurs as a result of infection with a unicellular parasite belonging to the genus Leishmania.
Diagnosis by conventional methods is inaccurate and is not sensitive to confirm the genus infection.
Here, we have investigated a methods for Leishmania genus diagnosis, which includes the technique of polymerase chain reaction to detect the presence of the parasite at in vitro for promastigote cultures using three genus-specific primer pairs to amplify HSP70, ITS, and ITS2.
The results showed single band of ~1422, ~1020, and ~550 respectively.
This study has proved the ability of these primer pairs to detect Leishmania infection and recommend them to be used for detection of leishmaniasis in hospitals and research centers.
American Psychological Association (APA)
Bayram, Duha Muhanad& al-Samirrai, Haydar Zuhayr Ali. 2017. Molecular detection of suspected leishmania isolates using polymerase chain reaction. Iraqi Journal of Science،Vol. 58, no. 4B, pp.2076-2082.
https://search.emarefa.net/detail/BIM-796556
Modern Language Association (MLA)
Bayram, Duha Muhanad& al-Samirrai, Haydar Zuhayr Ali. Molecular detection of suspected leishmania isolates using polymerase chain reaction. Iraqi Journal of Science Vol. 58, no. 4B (2017), pp.2076-2082.
https://search.emarefa.net/detail/BIM-796556
American Medical Association (AMA)
Bayram, Duha Muhanad& al-Samirrai, Haydar Zuhayr Ali. Molecular detection of suspected leishmania isolates using polymerase chain reaction. Iraqi Journal of Science. 2017. Vol. 58, no. 4B, pp.2076-2082.
https://search.emarefa.net/detail/BIM-796556
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-796556