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Direct detection of "methicillin resistant staphylococcus aureus" from buffalo raw milk in Al-Qadissiya province using polymerase chain reaction assay
Other Title(s)
التشخيص المباشر لبكتريا "المكورات العنقودية الذهبية المقاومة للمثيسيلين" من عينات حليب الجاموس الخام في محافظة القادسية باستخدام تقنية فحص تفاعل سلسلة البلمرة
Author
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 8, Issue 1 (30 Jun. 2017), pp.38-44, 7 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2017-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
The shedding of "methicillin resistant Staphylococcus aureus" in raw buffalo milk causes a potential risk if consumed without maintaining sufficient hygienic criteria due to numerous clinical implications to human infection.
The present study was used the Polymerase chain reaction technique as highly specific molecular procedures for direct detection of "methicillin resistant Staphylococcus aureus" (MRSA) of buffalo raw milk samples that compiled from animals owners of different areas in the province of Diwaniyah during the period from July 2016 to December 2016.
PCR technique was dependent on used specific primers that amplification of mecA gene in Staphylococcus aureus.
This primer was designed in this study by using NCBI-Genk data base (KM505043.1) and primer 3 plus for primers design.
The PCR results were shown that buffalo infected with (MRSA) at 8 positive samples at percent (16%) out of 50 milk samples.
The purpose of this research was to establish a rapid and specific PCR Technique for the diagnosis of "methicillin resistant Staphylococcus aureus" in buffalo raw milk that will be used as alternative to the currently available convention detection methods and makes it possible to identify the foods at risk for MRSA contamination to public health.
American Psychological Association (APA)
Ahmad, Hibah Shihab. 2017. Direct detection of "methicillin resistant staphylococcus aureus" from buffalo raw milk in Al-Qadissiya province using polymerase chain reaction assay. Kufa Journal for Veterinary Medical Sciences،Vol. 8, no. 1, pp.38-44.
https://search.emarefa.net/detail/BIM-797392
Modern Language Association (MLA)
Ahmad, Hibah Shihab. Direct detection of "methicillin resistant staphylococcus aureus" from buffalo raw milk in Al-Qadissiya province using polymerase chain reaction assay. Kufa Journal for Veterinary Medical Sciences Vol. 8, no. 1 (2017), pp.38-44.
https://search.emarefa.net/detail/BIM-797392
American Medical Association (AMA)
Ahmad, Hibah Shihab. Direct detection of "methicillin resistant staphylococcus aureus" from buffalo raw milk in Al-Qadissiya province using polymerase chain reaction assay. Kufa Journal for Veterinary Medical Sciences. 2017. Vol. 8, no. 1, pp.38-44.
https://search.emarefa.net/detail/BIM-797392
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 43-44
Record ID
BIM-797392