Molecular detection of haemotropic mycoplasma infection in sheep
Other Title(s)
الكشف الجزيئي عن الإصابة بالمايكوبلازما الدموية في الأغنام
Author
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 8, Issue 1 (30 Jun. 2017), pp.120-129, 10 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2017-06-30
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Abstract EN
This study was the first report about hemotropic mycoplasmoses in sheep at middle of Iraq as well as the first trail to investigate this infection among ticks .EDTA blood samples were collected from four hundred ( 400 ) Awassi sheep during February – July \2016 ,two hundard ( 200) of sheep clinicallyhad signs of anemia ,weakness ( infected group )and another 200 sheep that clinically healthy( healthy group ) ,all examined sheep were ranked according to gender( male and female ) and to age periods ( less than and more than one year ) and each sub group included 100 sheep , that tested by PCR assay by using specific Mycoplasma ovis primer with 1341 bp that detect infection rates 17.5% which significantly higher in infected group 25.5% than healthy group 7.5% and the yearling sheep was more susceptible to infection 22.5% than adult 12.5% and the infection was higher in female 20.4% than in male 14.5% , the Mycoplasma ovis infection was recorded with higher percentage in July 24.7% while no infection in February will be showed .
All blood samples of positivity infected sheep (70 ) were submitted into blood indices analysis which exhibited significant reduction in all blood parameters ( RBCs ,Hb ,PCV ,MCH ,MCHC, platelets ) when compare with group of negativity PCR healthy sheep as control group .
The trail to investigate about Mycoplasma ovis in (40) ticks picked up from examined sheep from tail and ear sites with standard parasitological methods by using the same primer of Mycoplasma ovis by PCR , this technique was gave negative result .
The conclusion include Mycoplasma ovis infection was registered in Awassi sheep with increasable rate in emaciated ,anemic sheep and so in yearling ,female and warm months of year with haemolytic macrocytic anemia.
American Psychological Association (APA)
Kashshash, Qasim Halim. 2017. Molecular detection of haemotropic mycoplasma infection in sheep. Kufa Journal for Veterinary Medical Sciences،Vol. 8, no. 1, pp.120-129.
https://search.emarefa.net/detail/BIM-797435
Modern Language Association (MLA)
Kashshash, Qasim Halim. Molecular detection of haemotropic mycoplasma infection in sheep. Kufa Journal for Veterinary Medical Sciences Vol. 8, no. 1 (2017), pp.120-129.
https://search.emarefa.net/detail/BIM-797435
American Medical Association (AMA)
Kashshash, Qasim Halim. Molecular detection of haemotropic mycoplasma infection in sheep. Kufa Journal for Veterinary Medical Sciences. 2017. Vol. 8, no. 1, pp.120-129.
https://search.emarefa.net/detail/BIM-797435
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 127-129
Record ID
BIM-797435