Comparative the different solvents and spad chlorophyll meter for determination some photosynthesis pigments of bean and cowpea plants

Abstract EN

In this work, some photosynthetic pigments with acetone, methanol and diethyl either were extracted spectrophotometrically, in addition to SPAD chlorophyll meter frombean(Phaseolus vulgaris L.) var.

Biotack and cowpea (VignasinensisSavi) var.

California black eye leaves in vegetative, flowering and podding growth stagescultivatedin Koya city, Erbil- Iraq.Results shows that concentration of chlorophyll a in bean and cowpea leaves increased significantly when acetone used in the extraction process followed by methanol and the lowest was obtained when the diethyl either was used, whereas methanol was the best solvent for extracting chlorophyll b.The concentration of chlorophyll a and b increased significantly in the flowering stage compared to vegetative and podding stages in bean plants and compared to vegetative growth in cowpea plants, whereas there were non-significant differences in total carotenoids with changing growth stage.

Results also showed that there were non-significant differences in SPAD readings between different growth stages in both of bean and cowpea plants.

SPAD readings correlated high significantly with chlorophylls a and b concentrations, when acetone used as solvent in cowpea plants.