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Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from cow's and buffalo's soft cheese samples in Babylon province, using multiplex PCR technique
Other Title(s)
التحري الجزيئي عن ذيفانات الاشريشية القولونية E.Coli O157:H7 و المعزولة من عينات الجبن المحلي للأبقار و الجاموس في محافظة بابل بتقنية PCR
Joint Authors
al-Isawi, Amir Jabbur Abayis
Najm, Najm Hadi
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 8, Issue 1 (30 Jun. 2017), pp.199-207, 9 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2017-06-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
Colonies of E.Coli O157:H7 were isolated from 80 locally produced Cow's and Buffalo's soft cheese samples that were collected randomly at weekly intervals (5 samples/ week) from various retail markets in different locations of babylon province during two climatic periods(40 samples /species/season) where the first periad was extended from the begining of December 2015 to the end of February 2016 while the second period was extended from the begining of July to the end of September 2016.The identification of E.Coli O157:H7 isolates were confirmed based on their cultural, biochemical and seralogical characteristics using the commercial latex agglutination test kit and by duplex PCR technique.
Data revealed that there was a significant (P≤ 0.05) seasonal variation in the prevalence of E.Coli O157:H7 where all Cow's and Buffalo's soft cheese samples had significantly (P≤ 0.05) higher prevalence of E.Coli O157:H7 in summer season (50% and 40% respectively) than in winter season (25% and 15% respectively).It was found that all of the 80 bovine soft cheese samples had significantly (p<0.05) higher prevalence of E.Coli O157:H7 in summer (45%) than in winter (20%) seasons.
The results of the diagnosis of E.coli O157:H7 were confirmed by PCR assay which showed that,six culturing isolates serotype E.coli O157:H7 were postive by PCR assay and five of these isolates expressed gene of Stx1 whearse one isolate expressed gene of Stx2.
American Psychological Association (APA)
Najm, Najm Hadi& al-Isawi, Amir Jabbur Abayis. 2017. Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from cow's and buffalo's soft cheese samples in Babylon province, using multiplex PCR technique. Kufa Journal for Veterinary Medical Sciences،Vol. 8, no. 1, pp.199-207.
https://search.emarefa.net/detail/BIM-797920
Modern Language Association (MLA)
Najm, Najm Hadi& al-Isawi, Amir Jabbur Abayis. Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from cow's and buffalo's soft cheese samples in Babylon province, using multiplex PCR technique. Kufa Journal for Veterinary Medical Sciences Vol. 8, no. 1 (2017), pp.199-207.
https://search.emarefa.net/detail/BIM-797920
American Medical Association (AMA)
Najm, Najm Hadi& al-Isawi, Amir Jabbur Abayis. Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from cow's and buffalo's soft cheese samples in Babylon province, using multiplex PCR technique. Kufa Journal for Veterinary Medical Sciences. 2017. Vol. 8, no. 1, pp.199-207.
https://search.emarefa.net/detail/BIM-797920
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 205-207
Record ID
BIM-797920