Detection virulence factors of Klebsiella pneumonia from cattle by using PCR tec.
Other Title(s)
الكشف عن عوامل ضراوة الكلبسيلا الرئوية المعزولة من الأبقار باستخدام تقنية سلسلة تفاعل البلمرة
Joint Authors
Ismail, Jamilah Radi
al-Ubaydi, Janan Nazim Sadiq
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 8, Issue 1 (30 Jun. 2017), pp.231-239, 9 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2017-06-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
Identification of K.
pneumoniae was avaluated using conventional microbiological characteristics and molecular assays .
Milk specimens were collected from cattle suffered from clinical symptoms of mastitis from different farms of AL-Qadisyiah province.
Out of 45 samples obtained, 20 isolates ( 44.4 %) were detected as K.
pneumonia according to morphology of colonies and biochemical features.
Molecular detection of Klebsiella pneumoniae based 16S rRNA gene for determination two virulence factor genes(rmpA,magA) by using specific primer.These genes potent the pathogenesis of Klebisella pneumonia.
The primers were made in this study by using NCBI-GenBank and primer3 plus design online.
The primer is made by company in Korea (Bioneer).
Moleculer detection of isolates which give away specific PCR products of of 312bp for magA gene and 835bp for rmpA gene .
The magA and rmpA genes were amplified in six (30%)and five(25%) isolates out of 20 isolates of Klebsiella pneumonia.
American Psychological Association (APA)
Ismail, Jamilah Radi& al-Ubaydi, Janan Nazim Sadiq. 2017. Detection virulence factors of Klebsiella pneumonia from cattle by using PCR tec.. Kufa Journal for Veterinary Medical Sciences،Vol. 8, no. 1, pp.231-239.
https://search.emarefa.net/detail/BIM-797925
Modern Language Association (MLA)
Ismail, Jamilah Radi& al-Ubaydi, Janan Nazim Sadiq. Detection virulence factors of Klebsiella pneumonia from cattle by using PCR tec.. Kufa Journal for Veterinary Medical Sciences Vol. 8, no. 1 (2017), pp.231-239.
https://search.emarefa.net/detail/BIM-797925
American Medical Association (AMA)
Ismail, Jamilah Radi& al-Ubaydi, Janan Nazim Sadiq. Detection virulence factors of Klebsiella pneumonia from cattle by using PCR tec.. Kufa Journal for Veterinary Medical Sciences. 2017. Vol. 8, no. 1, pp.231-239.
https://search.emarefa.net/detail/BIM-797925
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 237-239
Record ID
BIM-797925