Effect of different concentrations of cytokinin and auxins on in vitro propagation of Paulownia tomentosa steud and zucc

Abstract EN

Paulownia tomentosa Steud.

is one of the fast growing tree species with a wide ecological niche range permitting it to occur in a various types of habitat.

In addition, this species has a multiple ecological and industrial uses for reforestation, urban area and biomedical purposes.

Recently, Paulownia trees were widely propagated by tissue culture methods and planted in cities due to its high importance for infiltration of the air and ornamental.

However, in Kurdistan Region this tree was imported and had a high cost.

Therefore, the major aim of this investigation is to reach a successful protocol for propagation via tissue culture by testing the effects of growth regulators on the multiplication and rooting stages.

The results of this study showed that the most of growth regulators (auxins and cytokinin) had positive effects on the number of leaves, shoot length and number of nodes as compared with the control.

Moreover, MS salt strength supplemented with different growth regulators had also a positive effect on both roots number and length as compared with the control.

It's worth noting that the half strength of MS medium had more effect in giving a higher number of roots than full MS strength.

The results of this study highlighted the importance of this technique's advantages for further future micropropagation projects to forest tree species.

Knowing that this protocol should be applicated to propagate other native and introduced tree species and applying further investigations to test other in vitro effective factors