Evaluation of Leishmania major infectivity preserved in rpmi1640 media containing human urine in vivo

Abstract EN

The activity of Leishmania parasite decrease through passage and preserved in cultural media.

The usage of cultural media containing 10% human urine instead of the fetal calf serum which is difficult to obtain and it is costing as well, maintained the parasite activity after passage it through this medium and with ten passages through induced experimental infection in laboratory animals for eight groups of mice and when it got the viscera (spleen) at day 30 after infection, it had been noticed that there is no statistical significance ( p<0.05) in the foot pad of the infected groups after the third passage to tenth passage through the cultural media compared to the infected control animals after first passage from parasite isolated from cultural medium made by fetal calf serum.

No significant difference had been noted (p <0.05) in the numbers of the parasite (LD bodies) in splenic impressions between infected eight groups as compared to the control animals and this indicated parasite inactivity despite passing it through the cultural medium.

The aim of this study was to provide alternative cheap available material for fetal calf serum that maintains the infectivity and vitality of the parasite during long term preservation in culture medium without reactivation after repeatedly passage, this is important in experimental researches that aim to find a vaccine or curing cases of the parasitic infection.