Urinary excretion of 11 dehydrothromboxane B2 in diabetic children relation to clinical and biochemical parameters

Abstract EN

Diabetes is the 6th most important cause of disability burden in Egypt.

Cardiovascular diseases are the leading cause of death in diabetics Oxidative stress is involved in 0- cell destruction and is recognized as a mediatoi in the development of macro vascular or cardiovascular complications in type 1 diabetes mellitus.

Products of arachidontc acid metabolism elicit inflammatory responses and diseases in diabetic children such as atherosclerosis.

Hyperglycemia induced activation of thromboxane pathway evidenced by increase urinary excretion of 11- dehydrothromboxane B2 (indicator of oxidative stress) This study measured urinary excretion of 11 dehydro- thromboxane B2 in 40 type 1 diabetic children (12.38+ 2.75) years and 40, age and gendei matched, healthy controls (10 88+ 3.23) years.

Mean urinary II dehydrothromboxane B2 concentrations showed statistical significant difference between diabetic group (1884.8+ 826.86 pg.

/mg creatinine) and controls (601.95+ 229.24 pg/mg creatinine, p<0.001).

Also, total cholesterol (183.75+ 30.47 versus 112.6+ 27.07 mg/dl, p<0.001), triglycerides (147.45+ 29.91 versus 73.08+ 13.3, p<0.001), HDL (34.8+ 4.95 versus 45.6+ 8.25 mg/dl, p<0.00l), LDL (120.9+ 30.5 versus 83 6+ 24.2 mg/dl, p<0.001), HbAlC (11.48+ 1.79 versus 5.37+ 0.59, p<0 001) and fasting C- peptide (0.33+ 0.14 versus 2.05+ 0.87, p<0.001) showed statistical significant difference between diabetic children and adolescents and healthy controls Our results showed also significant positive correlation between urinary 11- dehydrothromboxane B2 and HbAlc (r= 0.627, p= 0.012), triglycerides (r= 0.520, p= 0.047) and total cholesterol (r= 0.668, p= 0.007).

In conclusion, the increase of triglycerides and LDL- cholesterol levels in our study confirmed the dyslipidemia pattern in pediatric type 1 DM patients.

Our results confirmed that hyperglycemia induced activation of thromboxane pathway in type 1 diabetic children and adolescents as evidenced by increase urinary excretion of the indicator of oxidative stress status li- dehydrothromboxane B2.

Also, we showed a significant positive correlation between the urinary exaction of 1 - dehydrothromboxane B2 and the laboratory parameters of lipid metabolism.

Therefore, urinary 11- dehydrothromboxane B2 can be used as a potential noninvasive biomarker of dyslipidemia in type 1 diabetic children.