Molecular diagnosis of Chlamydia pneumoniae in coronary heart disease patients in Diyala city
Joint Authors
Abd Allah, Hana Naji
al-Azawi, Isra F.
Salih, Muhammad Abd al-Dayim
Source
Iraqi Journal of Biotechnology
Issue
Vol. 16, Issue 4 (31 Dec. 2017), pp.79-88, 10 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Abstract EN
Chlamydia pneumoniae has been highly associated with & implicated in the procoagulatory & inflammatory component of atherosclerosis.
The present study has been designed to detect C.
pneumoniae DNA and C.
pneumoniae IgG and IgM antibodies among Iraqi patients with coronary heart disease and the association between the bacterium and risk factors.
The study involved 71 patients diagnosed with (angina, myocardial infarction and atherosclerosis) and 30 healthy controls.
Out from 71 patients, 28.2% were smoking, 26.8% were diabetic and 45.1% were hypertensive.
Detection of anti- C.
pneumoniae IgM and IgG antibodies was done by Enzyme-Linked Immunosorbent assay.
C.
pneumoniae IgG antibodies were detected in 57.7% of patients and 14.6% of healthy controls, while seropositivity of C.pneumoniae IgM antibodies were detected in 9.9% patients.
DNA were extracted from blood samples and PCR was used for detection of the C.
pneumoniae genes.
Thirty DNA samples of the coronary heart disease patients were amplified by PCR using three genes 16SrRNA with 194bp and outer membrane protein (OMP) and were included as internal with 239bp and outer with 499bp both specific gene for C.
pneumoniae.
The results of all genes revealed that 10 patients (33.3%) were positive , while it was negative for all control group.
American Psychological Association (APA)
Abd Allah, Hana Naji& Salih, Muhammad Abd al-Dayim& al-Azawi, Isra F.. 2017. Molecular diagnosis of Chlamydia pneumoniae in coronary heart disease patients in Diyala city. Iraqi Journal of Biotechnology،Vol. 16, no. 4, pp.79-88.
https://search.emarefa.net/detail/BIM-811027
Modern Language Association (MLA)
Abd Allah, Hana Naji…[et al.]. Molecular diagnosis of Chlamydia pneumoniae in coronary heart disease patients in Diyala city. Iraqi Journal of Biotechnology Vol. 16, no. 4 (2017), pp.79-88.
https://search.emarefa.net/detail/BIM-811027
American Medical Association (AMA)
Abd Allah, Hana Naji& Salih, Muhammad Abd al-Dayim& al-Azawi, Isra F.. Molecular diagnosis of Chlamydia pneumoniae in coronary heart disease patients in Diyala city. Iraqi Journal of Biotechnology. 2017. Vol. 16, no. 4, pp.79-88.
https://search.emarefa.net/detail/BIM-811027
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 86-88
Record ID
BIM-811027