Study of mutant frequency hypoxanthine guanine phosphoribosyl transferase (HPRT)‎ gene in human lymphocyte for local samples of al-Tuwaitha region-Iraq

Abstract EN

The aim of this study was déterminé the gcnctic damage in human blood lymphocytes for a number of 60 apparcntly hcalthy peoplcs were living Eshtar village and Al -Tamecm région, near to the Iraqi Atomic Energy commission using Hypoxanthinc guaninc phosphoribosyl transferase (HPRT) mutation assay.

Samplcs includcd 30 pcoples from Eshtar village, aged (15-55 years) and 30 peoplcs from Al-Tamccm région, aged ( 18 - 58 years), and 20 pcoples aged ( 18 - 56 years) from other régions of Baghdad as a control group, was studied.

The results of the average mutation frcquency for HPRT (Mf-HPRT) gcnc showed a signifieant incrcasc (p<0.05) in the maies and fcmalcs of human lymphocyte in this région as compared with the control group.

In conclusion, the results indicatcd that there is a possibility of using the changes at the level of HPRT gcnc mutation as uscful biomarkers for the détection of human exposure to radiological and chcmical matcrials.