Molecular-based identification of polystoma integerrimum by 28S rDNA, phylogenetic and secondary structure analysis
Joint Authors
al-Marjan, Karwan S. N.
Koyee, Qaraman M. K.
Abd Allah, Shamall Muhammad Amin
Khailany, Rozhgar Abd Allah Muhammad
Source
Jordan Journal of Biological Sciences
Issue
Vol. 9, Issue 2 (30 Jun. 2016), pp.117-121, 5 p.
Publisher
The Hashemite University Deanship of Academic Research and Graduate
Publication Date
2016-06-30
Country of Publication
Jordan
No. of Pages
5
Main Subjects
Abstract EN
The present study was carried out to molecular identification, phylogenetic evolutionary and secondary structure prediction of the monogenean, Polystoma integerrimum which was isolated from amphibians Bufo viridis and B.
regularis, collected from various regions in Erbil City, Iraq.
After the morphological examination of the parasite, molecular identification and phylogenetic relationships were carried out using 28S ribosomal DNA (rDNA) sequence marker.
The result indicated that the query sequence (sample sequence) was 100% identical to this species of the parasite and the phylogenetic tree showed 97-99% relationships in the sequence of P.
integerrimum and 28S rDNA regions for other species of Polystoma.
In addition, the present finding was confirmed by the molecular morphometrics, according to the secondary structure of 28S rDNA region.
The topology analysis produced the same data as the acquired tree.
In conclusion, the primary sequence analysis showed the validity of P.
integerrimum.
Phylogenetic tree and secondary structure analysis can be considered as a valuable method for separating species of Polystoma.
American Psychological Association (APA)
Koyee, Qaraman M. K.& Khailany, Rozhgar Abd Allah Muhammad& al-Marjan, Karwan S. N.& Abd Allah, Shamall Muhammad Amin. 2016. Molecular-based identification of polystoma integerrimum by 28S rDNA, phylogenetic and secondary structure analysis. Jordan Journal of Biological Sciences،Vol. 9, no. 2, pp.117-121.
https://search.emarefa.net/detail/BIM-811693
Modern Language Association (MLA)
Koyee, Qaraman M. K.…[et al.]. Molecular-based identification of polystoma integerrimum by 28S rDNA, phylogenetic and secondary structure analysis. Jordan Journal of Biological Sciences Vol. 9, no. 2 (Jun. 2016), pp.117-121.
https://search.emarefa.net/detail/BIM-811693
American Medical Association (AMA)
Koyee, Qaraman M. K.& Khailany, Rozhgar Abd Allah Muhammad& al-Marjan, Karwan S. N.& Abd Allah, Shamall Muhammad Amin. Molecular-based identification of polystoma integerrimum by 28S rDNA, phylogenetic and secondary structure analysis. Jordan Journal of Biological Sciences. 2016. Vol. 9, no. 2, pp.117-121.
https://search.emarefa.net/detail/BIM-811693
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 120-121
Record ID
BIM-811693