التوصيف الجزيئي و تحديد القرابة الوراثية لفيروس الجناح المشوه الذي يصيب نحل العسل المنتشر في سورية

Abstract EN

This study was based on the evaluation of 240 beehives distributed in eight Syrian provinces.

Multiplex one-step RT-PCR confirmed the presence of Deformed wing virus in 100% of symptomatic hives and in 55% of asymptomatic hives.

Phylogenetic analysis showed high homology among Syrian isolates that reached 97.9-100%, and 98.7-100% for both amplified fragments 195 and 395 bp, respectively.

The variability of nucleotides sequence of the 195 bp fragment led to some variability in the amino acids sequences produced, whereas, the 395 bp fragment coded for identical amino acid sequences in spite of the variability in the nucleotide sequences among virus isolates.

This study revealed that the Deformed wing virus is widespread in Syria, with high genomic homology between Syrian and global isolates.

Keywords: Deformed wing virus, honeybee, phylogenetic tree, Syria.