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Impact of biotic and abiotic elicitation on morphology, growth, active constituents and antibacterial activity of solanum nigrum (L.) calli induced in vitro
Other Title(s)
تأثير التظهير الحيوي و غير الحيوي على الشكل الظاهري و النمو و المركبات الفعالة و النشاط الضد بكتيري لكالس نبات السولانم نيجروم المتكون في المعمل
Joint Authors
Husayn, Nida Ahmad
Ubayd, Fawziyah Abu Sari Ahmad
Husayn, Isam Abd al-Salam
Source
Egyptian Journal of Desert Research
Issue
Vol. 67, Issue 1 (30 Jun. 2017), pp.47-63, 17 p.
Publisher
Publication Date
2017-06-30
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Solanum nigrum (L.) is an important herbaceous medicinal plant belonging to family Solanaceae.
It is antiseptic, antibacterial, antidiuretic and anti-inflammatory.
In the present study, stem nodal segments of Solanum nigrum were used as explants to induce callus formation on Murashige and Skoog basal medium fortified with 30 g/l sucrose, solidified with 6 g/l agar and supplemented with BA and 2,4-D under biotic (autoclaved Fusarium oxysporum culture filtrate) and abiotic elicitation (mannitol induced osmotic stress).
Results revealed that the callus vigor of growth, color, texture and the nature of the surface of fresh and dry weight, size of callus and contents of some secondary metabolites were affected by the action and interaction and concentration of the two applied elicitors.
Elicitation, in general, enhanced the biosynthesis and accumulation of secondary metabolites in callus tissues.
Alkaloids were increased from 30 mg/g in dry callus powder (control) to 200 mg/g in calli treated with 1% fungal filtrate and 100 mM mannitol.
Flavonoids increased from 20 mg/g in dry callus powder (control) to 410 mg/g in calli treated with 1% fungal filtrate without mannitol.
Tannins increased from 10 mg/g in dry callus powder (control) to 310 mg/g in calli treated with 0.5% fungal filtrate and 50 mM mannitol or 1% fungal filtrate with or without 50 mM mannitol.
The obtained results indicated also that elicited calli exhibited in general much more pronounced antibacterial effect against the four tested pathogenic bacterial strains (Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli).
The obtained results indicate the possibility of maximizing the production of valuable phytopharmaceutics in vitro using biotic and abiotic elicitation.
American Psychological Association (APA)
Ubayd, Fawziyah Abu Sari Ahmad& Husayn, Isam Abd al-Salam& Husayn, Nida Ahmad. 2017. Impact of biotic and abiotic elicitation on morphology, growth, active constituents and antibacterial activity of solanum nigrum (L.) calli induced in vitro. Egyptian Journal of Desert Research،Vol. 67, no. 1, pp.47-63.
https://search.emarefa.net/detail/BIM-812605
Modern Language Association (MLA)
Ubayd, Fawziyah Abu Sari Ahmad…[et al.]. Impact of biotic and abiotic elicitation on morphology, growth, active constituents and antibacterial activity of solanum nigrum (L.) calli induced in vitro. Egyptian Journal of Desert Research Vol. 67, no. 1 (Jun. 2017), pp.47-63.
https://search.emarefa.net/detail/BIM-812605
American Medical Association (AMA)
Ubayd, Fawziyah Abu Sari Ahmad& Husayn, Isam Abd al-Salam& Husayn, Nida Ahmad. Impact of biotic and abiotic elicitation on morphology, growth, active constituents and antibacterial activity of solanum nigrum (L.) calli induced in vitro. Egyptian Journal of Desert Research. 2017. Vol. 67, no. 1, pp.47-63.
https://search.emarefa.net/detail/BIM-812605
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 59-62
Record ID
BIM-812605