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Comparative studies on infectious bursal disease virus propagated in chicken eggs and Vero cell culture
Other Title(s)
دراسات مقارنة علي فيروس التهاب غدة فابيرش المعدي المؤقلم على أجنة بيض الدجاج و خلايا كلى القرد الأفريقي
Joint Authors
Fawzi, Muhammad
al-Tarabili, Mukhtar Muhammad Ali
al-Misri, Hibah H.
Abd al-Wahhab, Shahira A.
Khudayr, Muhammad Hassan
Source
Suez Canal Veterinary Medicine Journal
Issue
Vol. 21, Issue 1 (30 Jun. 2016), pp.109-120, 12 p.
Publisher
Suez Canal University Faculty of Veterinary Medicine
Publication Date
2016-06-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Topics
Abstract EN
Ten bursal samples were pooled from the IBD infected birds in Damietta Governorate showing haemorrhagic and enlarged bursae.
Bursal homogenates were used for ten-day-old embryonated specific pathogen free embryonated tests included VN and FAT tests revealed the incidence of IBDV antigen.
Propagation of the obtained isolate in ECE and Vero cells for 10 passages revealed gradual increase in the virus titer up to 8 log10EID50/ml and 7.5TCID50/ml respectively.
Original, egg adapted, cell culture adapted and bursavac samples were used for viral RNA extraction by using reagent---- (Trizol).
RT-PCR was performed for samples using V1 and V2 pair of primers of hypervariable region of VP2 gene to confirm the presence of IBDV in cell culture.
The results of Viral RNA extracted from all samples is generation of a targeted amplicon of 472 bp confirming that there were no differences between any of them.
American Psychological Association (APA)
Khudayr, Muhammad Hassan& al-Tarabili, Mukhtar Muhammad Ali& al-Misri, Hibah H.& Abd al-Wahhab, Shahira A.& Fawzi, Muhammad. 2016. Comparative studies on infectious bursal disease virus propagated in chicken eggs and Vero cell culture. Suez Canal Veterinary Medicine Journal،Vol. 21, no. 1, pp.109-120.
https://search.emarefa.net/detail/BIM-812824
Modern Language Association (MLA)
Khudayr, Muhammad Hassan…[et al.]. Comparative studies on infectious bursal disease virus propagated in chicken eggs and Vero cell culture. Suez Canal Veterinary Medicine Journal Vol. 21, no. 1 (2016), pp.109-120.
https://search.emarefa.net/detail/BIM-812824
American Medical Association (AMA)
Khudayr, Muhammad Hassan& al-Tarabili, Mukhtar Muhammad Ali& al-Misri, Hibah H.& Abd al-Wahhab, Shahira A.& Fawzi, Muhammad. Comparative studies on infectious bursal disease virus propagated in chicken eggs and Vero cell culture. Suez Canal Veterinary Medicine Journal. 2016. Vol. 21, no. 1, pp.109-120.
https://search.emarefa.net/detail/BIM-812824
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 117-119
Record ID
BIM-812824