Frequency of antiseptic resistance among staphylococcus aureus and coagulase-negative staphylococci isolated from a university hospital in central Iran
Joint Authors
Taheri, Nona
Ardebili, Abd Allah
Amouzandeh Nobaveh, Alireza
Ghaznavi Rad, Ihsan Allah
Source
Issue
Vol. 31, Issue 6 (30 Nov. 2016), pp.426-432, 7 p.
Publisher
Publication Date
2016-11-30
Country of Publication
Oman
No. of Pages
7
Main Subjects
Abstract EN
Objectives: Reduced biocide susceptibility in Staphylococci is associated with various antiseptic resistance genes encoding efflux systems.
Our aim was to determine the susceptibility to three disinfectant agents, including benzalkonium chloride (BABAC), benzethonium chloride (BZT), and chlorhexidine digluconate (CHDG) among clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococci (CoNS).
Methods: The minimum inhibitory concentration (MIC) of 60 methicillin-resistant S.
aureus (MRMRSA), 54 methicillin-sensitive S.
aureus (MSSA) and 51 CoNS isolates from a single hospital to three biocidal agents (BABAC, BZT, and CHDG) was determined.
Biocide resistance genes (qacA/B, smr, qacG, qacH, qacJ, and norA) were analyzed by the polymerase chain reaction assay.
Results: All isolates had MICs for BABAC and BZT from 0.25 to 8 μg/mL, and for CHDG from 0.5 to 64 μg/mL.
qacA/B was the most common biocide resistance gene among all 165 Staphylococcus isolates (76; 46%), which comprised 38 (63.3%) MRMRSA, 14 (25.9%) MSSA, and 24 (47%) CoNS.
Eleven (6.7%) and 24 (14.5%) isolates among the 165 Staphylococci carried smr and norA genes, respectively.
In contrast, other resistance genes such as qacG, qacH, and qacJ were absent in all Staphylococci studied.
The qacA/B and smr genes were detected concomitantly in 3% of isolates, and 23.6% strains of the total 165 Staphylococcus isolates were negative for each studied gene.
Conclusions: The carriage of several biocide resistance genes, including qacA/B, smr, and norA, alone or concurrently, is associated with reduced susceptibility.
Use of antiseptics may select for antibiotic-resistant strains and assist their survival in the healthcare environment
American Psychological Association (APA)
Taheri, Nona& Ardebili, Abd Allah& Amouzandeh Nobaveh, Alireza& Ghaznavi Rad, Ihsan Allah. 2016. Frequency of antiseptic resistance among staphylococcus aureus and coagulase-negative staphylococci isolated from a university hospital in central Iran. Oman Medical Journal،Vol. 31, no. 6, pp.426-432.
https://search.emarefa.net/detail/BIM-813245
Modern Language Association (MLA)
Taheri, Nona…[et al.]. Frequency of antiseptic resistance among staphylococcus aureus and coagulase-negative staphylococci isolated from a university hospital in central Iran. Oman Medical Journal Vol. 31, no. 6 (Nov. 2016), pp.426-432.
https://search.emarefa.net/detail/BIM-813245
American Medical Association (AMA)
Taheri, Nona& Ardebili, Abd Allah& Amouzandeh Nobaveh, Alireza& Ghaznavi Rad, Ihsan Allah. Frequency of antiseptic resistance among staphylococcus aureus and coagulase-negative staphylococci isolated from a university hospital in central Iran. Oman Medical Journal. 2016. Vol. 31, no. 6, pp.426-432.
https://search.emarefa.net/detail/BIM-813245
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 432
Record ID
BIM-813245