Antifertility activity of Melia azedarach in male rabbits

Abstract EN

This study was conducted on 10 albino adult male rabbits, of 1-2 kg b.

wt., 1-2 years old.

The animals were divided in two groups, treated group was treated with Melia azedarach in powdered form at a dose rate of 6 g / kg b.wt.

orally mixed with feed daily for 53 days.

While those of control group were left without treatment.

All animals were killed at 56 days of experiment Blood samples were collected to obtain the serum for biochemical examination.

In addition to weight the testes, head, body, tail of epididymis, seminal vesicle and prostate.

Total number of sperms in epididymis head was accounted.

Calculation of life sperm and deformed sperms.

The dependent parameters were included clinical and hematological examination.

The results of the study revealed that in treated group clotting time was significantly decreased.

While bleeding time was non significantly increased in 23rd day.

Erythrocytes counts, Hb concentration, PCV%; MCH were non significantly increased.

MCV increased in day 23rd but decreased in 56th day.

Meanwhile MCHC non significantly decreased.

Total leucocytes count was not changed in both treated and control groups.

lymphocyte percent in treated group was non significantly increased, while Heterophils and eosinophils percent were non significantly decreased.

Basophils and Monocytes percentages were significantly decreased.

The results revealed that the weight of sexual organs and the accessory glands, in addition to total sperm counts of treated males were less than those of control males.

While the dead sperm and deformed sperm were more in treated than in control males.

The results revealed that ALT, AST and AP values were within normal range but of lower level in treated males in comparison with those of control males.

Histopathological examinations revealed that the main changes in sexual organs of treated male were included, appearance of spermatid giant cells with few sperms in the lumen of seminiferous tubules, round spermatid cells in the lumen of seminiferous tubules with vacuolation of their epithelial cells.

cellular debris in the lumen of epididymis with vacuolation of the epithelial lining.

While in control males the main histological changes in sexual organs were included sperms filled the lumen of epididymis, and normal structure.

In the testis shows complete spermatogenesis with sperms in the seminiferous tubules.

In seminiferous tubules shows normal structure and their lumen contain sperms.

From results we can conclude that Melia azedarach has an anticonception material that effect the total number of sperm with inducing a deformity in sperm parts, with less side effects on health of treated males