In vitro capacitation of buck spermatozoa

Abstract EN

Testes of adult Buck (n=15) were taken from Al-Fallouja slaughter house and transferred with cool box containing normal saline to the lab of Vet.

Med.

College, Al-Anbar University during the period from October 2013 to December 2013 within 30 minutes.

Semen were collected from the tail of epididymis by aspiration with 3ml (18-guage) disposable syringe containing 1-2ml of MEM media.

It's also the tail of epididymis subjected to several incisions with a sharp surgical blade and applying a pressure at the base of epididymis to collect the remnant of spermatozoa.

The collected materials were put in a sterile petridish and incubated at 35ₒc.

samples were examined to evaluate the degree of sperm's maturation.

The semen then incubated in 5% co2, 35ₒc with 90% relative humidity for 4-6 hours.

After maturation of spermatozoa the suspension was diluted 1:1 with heparin (100µg/ml heparin salt) and incubated for 15 minutes at 5% Co2, 35ₒc with 90% relative humidity.

Head to Head agglutination of spermatozoa was considered to be a criteria of sperm capacitation.

The result showed the possibility of sperm maturation obtained from cauda epididymis of Buck in MEM media in vitro.

The presence of distal protoplasmic droplet was the criteria of spermatozoa maturation.

It was concluded from this study that it's possible of sperm maturation and capacitation taken from tail of epididymis of buck from slaughter house source in vitro