Determination of alanine, arginine, and tyrosine in black, oolong, green and white tea by derivation with ortho-phthalaldehyde using HPLC-FLD
Other Title(s)
تحديد الألانين و الأرجنين و التيروزين في عينات من الشاي الأسود و الأولنج و الأخضر و الأبيض بالتحليل الكروماتوغرافي السائل عالي الأداء المزود بكاشف الفلورة عن طريق الاشتقاق بأورتو فتال ألدهيد
Joint Authors
Source
Tishreen University Journal for Research and Scientific Studies : Basic Sciences Series
Issue
Vol. 36, Issue 3 (30 Jun. 2014), pp.137-148, 12 p.
Publisher
Publication Date
2014-06-30
Country of Publication
Syria
No. of Pages
12
Main Subjects
Abstract EN
A simple gradient HPLC method utilise a fluorescence detector (FLD) was developed for highly sensitive, rapid and reliable quantitative determination of Alanine, Arginine, and Tyrosine found mainly in commercial tea types of black, oolong, green, and white.
The method depends on the derivation of amino acids, with a mixture of O-Phthalaldehyde and 3-Mercaptopropionic acid reagents.
The chromatographic separation was achieved using a reversed-phase Agilent Zorbax Exclipe RP18 (C18), 250 mm × 4.6 mm, 5 μm column depending on the gradient HPLC method employs in two solvents A and B as mobile phase.
Solvent A contains a mixture of tetrahydrofuran: acetate buffer pH 7.6 (3:97, v/v), and solvent B contains a mixture of acetate buffer pH 7.6 : acetonitrile : methanol (1:2:2,v/v/v).
The flow rate of mobile phase was 1.5 mL/min, The FL detector was set at excitation wavelength (λEx) of 340 nm and emission wavelength (λ Em) of 450 nm.
The calculated detection limit LOD for amino acids were very low, in the range of 1.214 – 5.592 nmol/L, with very low limit of quantification LOQ range of 3.680 – 16.945 nmol/L and RSD range 1.05 – 2.87%.
The developed RP-HPLC method was validated with respect to accuracy, precision and was shown advantageous over the simpler methodology with highly precision, accuracy, sensitive, and negligible interference of natural present in tea infusion.
The proposed method was successfully applied for the separation and determination of Alanine, Arginine, and Tyrosine in eleven tea samples of black, oolong, green and white found in the local market.
American Psychological Association (APA)
Sadiq, Hassan& Qazan, Rana. 2014. Determination of alanine, arginine, and tyrosine in black, oolong, green and white tea by derivation with ortho-phthalaldehyde using HPLC-FLD. Tishreen University Journal for Research and Scientific Studies : Basic Sciences Series،Vol. 36, no. 3, pp.137-148.
https://search.emarefa.net/detail/BIM-815899
Modern Language Association (MLA)
Sadiq, Hassan& Qazan, Rana. Determination of alanine, arginine, and tyrosine in black, oolong, green and white tea by derivation with ortho-phthalaldehyde using HPLC-FLD. Tishreen University Journal for Research and Scientific Studies : Basic Sciences Series Vol. 36, no. 3 (2014), pp.137-148.
https://search.emarefa.net/detail/BIM-815899
American Medical Association (AMA)
Sadiq, Hassan& Qazan, Rana. Determination of alanine, arginine, and tyrosine in black, oolong, green and white tea by derivation with ortho-phthalaldehyde using HPLC-FLD. Tishreen University Journal for Research and Scientific Studies : Basic Sciences Series. 2014. Vol. 36, no. 3, pp.137-148.
https://search.emarefa.net/detail/BIM-815899
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-815899