Antihyperglycemic and antiadhesion activity of gallic acid in induced urinary tract infection in diabetic rats

Abstract EN

This study was undertaken to investigate the role of gallic acid in attenuating hyperglycemia and inhibiting bacterial adhesion to bladder epithelial cells in induced diabetes and urinary tract infection in rats.

Diabetes was induced in rats by intraperitoneal injection of alloxan.

Blood glucose levels were measured daily and glucose levels of >250 mg/dl was considered as positive for diabetes initiation.

Bladder infection was induced by transurethral catheterization of Escherichia coli.

Gallic acid was administered orally at a dose of 2.5,5 and 10 mg/kg and compared with a control group and a group administered ciprofloxacin orally (7.2 mg/kg).

Blood glucose level was measured after induced E.coli infection in the rats of each group then the rats were sacrificed and the bladders were taken for histopathology and for the determination of adhesion of E.coli to bladder epithelium.

The study demonstrated that gallic acid at concentrations of 2.5, 5 and 10 mg/kg significantly (P ≤0.05) reduced elevated blood sugar and inhibited the adhesion of E.coli to bladder epithelial cells compared to controls.

Histological changes revealed decrease in severity of acute inflammation and edema with decreased number of neutrophils invading epithelial cells compared to diabetic group