Effect of growth regulators and ecotypes of egyptian vinca rosa (Catharanthus roseus) on callus induction and alkaloids production
Other Title(s)
تأثير منظمات النمو و الطرز البيئية لنبات الونكا المصري على استحداث الكالس و إنتاج القلويدات
Joint Authors
Ismail, Tariq Abu al-Mahasin
Gharib, Yasir Izzat
Gharib, Yasir Izzat
Sulayman, Said Sad A.
Hasan, Muhammad Abu Bakr
Source
Zagazig Journal for Agricultural Researches
Issue
Vol. 43, Issue 1 (28 Feb. 2016), pp.141-150, 10 p.
Publisher
Zagazig University Faculty of Agriculture
Publication Date
2016-02-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Topics
- Plant genetics
- Agriculture
- Egypt
- Twenty-first century
- Plant cell culture
- Growth
- Catharanthus roseus
- Growth regulators
Abstract EN
Objective of the study is to estimate the effect of growth regulators and different ecotypes of Egyptian Catharanthus roseus such as (Ismailia, Fayoum, Alexandria, Sharkia and Cairo) on callus induction and alkaloids production.
Different growth regulators combinations were applied.
Murashige and Skoog (MS) medium supplemented with four different growth regulators, i.e.
M1 (2, 4- Dichlorophenoxy acetic acid (2, 4-D) 1.0 μM and Benzyl adenine (BA) 0.5 μM), M2 (2, 4-D 0.5 μM and BA 1.0 μM), M3 (BA 1.0 μM and 1-Naphthaleneacetic acid (NAA) 0.5 μM) and M4 (BA 0.5 μM and NAA 1.0 μM).
M1 medium gave the highest value for callus induction frequencies (75%) and callus fresh weight (1.87 g) for produced callus from leaf while the stem produced callus (0.7 g) was the higher too than other mediums.
The total alkaloids content (TAC) was estimated using spectrophotometer with Bromocresol green (BCG).
The results showed that TAC outperformed in produced callus from stem explant than leaf produced callus, leaf and stem of original plant.
The important result showed that the Fayoum ecotype was the highest alkaloid content (2.22 mg/g) for produced callus from stem explant than all studied ecotypes.
American Psychological Association (APA)
Gharib, Yasir Izzat& Gharib, Yasir Izzat& Sulayman, Said Sad A.& Ismail, Tariq Abu al-Mahasin& Hasan, Muhammad Abu Bakr. 2016. Effect of growth regulators and ecotypes of egyptian vinca rosa (Catharanthus roseus) on callus induction and alkaloids production. Zagazig Journal for Agricultural Researches،Vol. 43, no. 1, pp.141-150.
https://search.emarefa.net/detail/BIM-816721
Modern Language Association (MLA)
Gharib, Yasir Izzat…[et al.]. Effect of growth regulators and ecotypes of egyptian vinca rosa (Catharanthus roseus) on callus induction and alkaloids production. Zagazig Journal for Agricultural Researches Vol. 43, no. 1 (2016), pp.141-150.
https://search.emarefa.net/detail/BIM-816721
American Medical Association (AMA)
Gharib, Yasir Izzat& Gharib, Yasir Izzat& Sulayman, Said Sad A.& Ismail, Tariq Abu al-Mahasin& Hasan, Muhammad Abu Bakr. Effect of growth regulators and ecotypes of egyptian vinca rosa (Catharanthus roseus) on callus induction and alkaloids production. Zagazig Journal for Agricultural Researches. 2016. Vol. 43, no. 1, pp.141-150.
https://search.emarefa.net/detail/BIM-816721
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-816721