Isolation and partial characterization of two bacteriophages virulent to Enterobacter cloacae
Other Title(s)
عزل و توصيف جزئي لاثنين من البكتيريوفاجات الضارية لبكتريا الانتيروباكتر كلوأسي
Joint Authors
Hijazi, Muhammad Ibrahim
Ilzamik, Fatimah Ibrahim
Ahmadi, Manar Muhammad Jamal
al-Wafai, Nahid Amin
Source
Zagazig Journal for Agricultural Researches
Issue
Vol. 43, Issue 2 (30 Apr. 2016), pp.423-434, 12 p.
Publisher
Zagazig University Faculty of Agriculture
Publication Date
2016-04-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Topics
Abstract EN
Enterobacter cloacae is a potential bacterial pathogens in aquatic water .
In this study, two lytic phages designated as Ec02 and Ec03 infectious to Enterobacter cloacae were isolated from agricultural irrigation water.
Morphological analysis by transmission electron microscopy revealed that both phages belongs to Myoviridae family.
Phage Eco2 had an isometric head 92 nm in diameter with a long contractile tail 125 nm in length, while phage Ec03 had a head of about 75 nm in diameter with a long contractile tail 92 nm in length.
Both phages were shown to be virulent to different Enterobacter sp isolates except Enterobacter aerogenes (57), and had no effect on other tested bacteria.
One- step growth curves revealed that the latent period, rise periods and burst size of phages Ec02 and Ec03 were (20, 20 min) , (30,40 min) and (146, 94 PFU/ infected cell), respectively .
Both phages were thermal stable and were tolerant to a wide range of pH.
These results suggest that both phages have a high potential for phage application to control Enterobacter cloacae.
American Psychological Association (APA)
Ahmadi, Manar Muhammad Jamal& Ilzamik, Fatimah Ibrahim& al-Wafai, Nahid Amin& Hijazi, Muhammad Ibrahim. 2016. Isolation and partial characterization of two bacteriophages virulent to Enterobacter cloacae. Zagazig Journal for Agricultural Researches،Vol. 43, no. 2, pp.423-434.
https://search.emarefa.net/detail/BIM-816749
Modern Language Association (MLA)
Ahmadi, Manar Muhammad Jamal…[et al.]. Isolation and partial characterization of two bacteriophages virulent to Enterobacter cloacae. Zagazig Journal for Agricultural Researches Vol. 43, no. 2 (2016), pp.423-434.
https://search.emarefa.net/detail/BIM-816749
American Medical Association (AMA)
Ahmadi, Manar Muhammad Jamal& Ilzamik, Fatimah Ibrahim& al-Wafai, Nahid Amin& Hijazi, Muhammad Ibrahim. Isolation and partial characterization of two bacteriophages virulent to Enterobacter cloacae. Zagazig Journal for Agricultural Researches. 2016. Vol. 43, no. 2, pp.423-434.
https://search.emarefa.net/detail/BIM-816749
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-816749