Effect of gibberellic acid on strawberry (Fragaria X ananassa Duch.)‎ micropropagation during multiplication stage

Abstract EN

-The present work was conducted at the tissue culture lab.

of the Center of Genetic Engineering and Biotechnology, Fac.

Agric., Ain Shams University, Egypt.

It aims to reveal the micropropagation possibilities of Festival and Marquez strawberry cultivars using runners meristem culture on different concentrations of gibberellic acid (GA3); i.e., 0.1, 0.2, 0.3, 0.4 and 0.5 mgl-1) in multiplication stage.

Supplementation the media with GA3 at 0.4 mgl-1 gave the highest values for number of shoots per explant and leaf number per shoot, whereas GA3 at 0.5mgl-1for shoot length was found to be the most appropriate concentration.

In addition, Festival strawberry cultivar achieved the best results of all features under study than Marquez cultivar.

The most favourble interaction treatment for maximizing number of shoots/explant and number of leaves/shoot was MS medium containing 0.1 mgl-1 benzyladenine (BA) + 0.4 mgl-1 GA3 with Festival strawberry cultivar