![](/images/graphics-bg.png)
In vitro relationship between organogenesis from Datura metel L. anther and catalase activity
Other Title(s)
العلاقة ببن نشوء الأعضاء من متك الداتورة Datura metel L. و فعالية الكاتليز خارج الجسم الحي
Author
Source
Zagazig Journal for Agricultural Researches
Issue
Vol. 43, Issue 4 (31 Aug. 2016), pp.1173-1179, 7 p.
Publisher
Zagazig University Faculty of Agriculture
Publication Date
2016-08-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The aim of the present study was to investigate the effect of different concentrations of plant growth regulators on in vitro induction of callus and plantlets regeneration from anthers culture of Datura metel L.
The activity of catalase (CAT) as one of the important antioxidant enzymes was measured during different stages of callus and organogenesis under all combinations of hormones.
Anthers were cultured under aseptic conditions on Murashige and Skoog (MS) medium supplemented with benzyladenine (BA) (0.0, 3.0, 5.0 and 7.0 mg/l) in combination with 2,4-D (2,4-dichlorophenoxy acetic acid) (0.0, 0.01 or 0.05 mg/l) for callus induction and organogenesis.
Results showed that MS containing different combinations of BA and 2,4-D induced calli with percentage ranging between 20- 100%.
High concentrations of growth regulators enhanced the highest callus percentage and promoted plantlets regeneration by indirect organogenesis reached to higher numbers of regenerated plantlets (4.5 and 7.4) at 5.0 mg/l BA + 0.05mg/l 2,4-D and 7.0 mg/l BA + 0.05 mg/l 2,4-D, respectively.
Results revealed that catalase activity increased significantly in organogenic callus with regenerated plantlets (23.5 and 47.3 unit) at higher concentrations of BA and 2,4-D (5.0 mg/l BA + 0.05 mg/l 2,4-D and 7.0 mg/l BA + 0.05 mg/l 2,4-D, respectively).
American Psychological Association (APA)
Salim, Siham Abd al-Razzaq. 2016. In vitro relationship between organogenesis from Datura metel L. anther and catalase activity. Zagazig Journal for Agricultural Researches،Vol. 43, no. 4, pp.1173-1179.
https://search.emarefa.net/detail/BIM-816950
Modern Language Association (MLA)
Salim, Siham Abd al-Razzaq. In vitro relationship between organogenesis from Datura metel L. anther and catalase activity. Zagazig Journal for Agricultural Researches Vol. 43, no. 4 (2016), pp.1173-1179.
https://search.emarefa.net/detail/BIM-816950
American Medical Association (AMA)
Salim, Siham Abd al-Razzaq. In vitro relationship between organogenesis from Datura metel L. anther and catalase activity. Zagazig Journal for Agricultural Researches. 2016. Vol. 43, no. 4, pp.1173-1179.
https://search.emarefa.net/detail/BIM-816950
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-816950