Stability of Staphylococcus aureus biofilm against different chemical agents and some enzymes

Abstract EN

The Biofilm of Staphylococcus aureus was tested against a various of substances at different concentrations for ١٢ hr., to show its stability.

The chelating agent Ethylene diamine tetra acetic acid (EDTA) (١-٥%) didn't show any effect on the biofilm stability.

Oxidizing agents also didn`t show a negative effect on the biofilm stability.

By adding enzymes like Pepsin and Papain (١٠٠-٥٠٠μl) the stability of biofilm did not affect.

Organic solvents e.g.; hexan, toluene and methyl alchohol added at ١-٥% also did not destabilize the biofilm stability.

Acids and basics like (HCl and NaOH) were added with concentrations of ١-٥%.

The effect of different substance on the stability of biofilm were very slight.

Adjusting the bacterial media to pH ٢-١٣ using HCl and NaOH before the biofilm formation was effected and showed positive effect and prevent biofilm formation between pH (٢-٥) and pH (١٠-١٣).