Application of PCR-based markers for off-type detection in rice CMS lines

Abstract EN

The cytoplasmic male sterile (CMS) lines that are used in production of hybrid seeds in three-line system often get contaminated with their maintainer lines.

Using such impure CMS lines in rice hybrid seed production lead to un-uniformity and yield loss in hybrid rice.

Laborious and time consuming phenotypic evaluations are used for elimination of off-type plants in CMS multiplication farms.

The other option for off-type elimination in CMS lines is application of molecular markers.

In this study, PCR-based markers were used for distinguishing five male sterile lines from other five maintainer lines.

Results showed that cms marker had ability to differentiate male sterile lines Neda A and Nemat A from their maintainers.

Also, drrcms marker differentiated male sterile lines Dasht A, Champa A and Amol-3 A from their respective maintainers.

Results of the study demonstrate the use of these two markers for off-type detection in CMS seed multiplication process.

This is helpful in promotion of hybrid seed production technology in Iran.