Extraction strategy for DNA recovery from putrefied teeth and skull bone
Other Title(s)
استراتيجية استخلاص للحصول على الحمض النووي من أنسجة صلبة متحللة (الأسنان و عظام الجمجمة)
Joint Authors
Kamoun, Arwa
Bin Ammar, Wiem
Mahfuz, Nadiyah
Maalej, Layla
Mallek, Bakhta
Nimah, Yusuf
Barda, Sami
Makni, Hafiz
Matuq, Samir
Source
Arab Journal of Forensic Sciences and Forensic Medicine
Issue
Vol. 1, Issue 4 (31 Dec. 2016), pp.399-405, 7 p.
Publisher
Naif Arab University for Security Sciences Arab Society for Forensic Sciences and Forensic Medicine
Publication Date
2016-12-31
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Economics & Business Administration
Abstract EN
Forensic samples are commonly exposed to harsh environmental conditions which affect the degree of sample (DNA) preservation and subsequent genetic profiling.
The aim of this study was to develop a better strategy for DNA extraction from hard putrefied tissues (Teeth and Skull bone).
Jaw (teeth) and the skull samples were collected from the putrefied corpses and the authors were asked to determine if the two specimens belonged to the same body.
The DNA was extracted by phenol-chloroform and DNA IQ™ System Kit.
The PowerPlex 16 and the PowerPlex® Y System Kits were used for autosomal STR and Y-STR genotyping, respectively.
DNA profiling found evidence in favor of DNA degradation.
Phenol-Chloroform extracted-DNA was re-extracted by using DNA IQ ™ System kit and managed to identify 13 autosomal STR loci and 13 Y-STR markers from doubly extracted DNA.
In conclusion, the combination of two DNA extraction methods (phenol-chloroform + DNA IQ™) improved the quality of DNA extracted from putrefied teeth and skull bone.
American Psychological Association (APA)
Kamoun, Arwa& Bin Ammar, Wiem& Mahfuz, Nadiyah& Maalej, Layla& Mallek, Bakhta& Nimah, Yusuf…[et al.]. 2016. Extraction strategy for DNA recovery from putrefied teeth and skull bone. Arab Journal of Forensic Sciences and Forensic Medicine،Vol. 1, no. 4, pp.399-405.
https://search.emarefa.net/detail/BIM-818810
Modern Language Association (MLA)
Kamoun, Arwa…[et al.]. Extraction strategy for DNA recovery from putrefied teeth and skull bone. Arab Journal of Forensic Sciences and Forensic Medicine Vol. 1, no. 4 (Dec. 2016), pp.399-405.
https://search.emarefa.net/detail/BIM-818810
American Medical Association (AMA)
Kamoun, Arwa& Bin Ammar, Wiem& Mahfuz, Nadiyah& Maalej, Layla& Mallek, Bakhta& Nimah, Yusuf…[et al.]. Extraction strategy for DNA recovery from putrefied teeth and skull bone. Arab Journal of Forensic Sciences and Forensic Medicine. 2016. Vol. 1, no. 4, pp.399-405.
https://search.emarefa.net/detail/BIM-818810
Data Type
Journal Articles
Language
English
Record ID
BIM-818810