Molecular localization of human papilloma virus genotypes (16, 18, and 611)‎ in patients with colorectal cancer by DNA-Insitu hybridization

Other Title(s)

الموضعه الجزيئية للفيروس الحليمي البشري ذو الأنماط الجينية (16 و 18 6 و 11)‎ في مرضى سرطان القولون و المستقيم باستخدام تقنيه الدنا التهجين الموضعي

Joint Authors

Ali, Sad Hasan Muhammad
Jasim, Balqis Sadun
al-Wakil, Nizar Abd al-Hasan
Dawud, Dawud Salman

Source

Iraqi Journal of Cancer and Medical Genetics

Issue

Vol. 8, Issue 1 (30 Jun. 2015), pp.14-20, 7 p.

Publisher

al-Mustansyriah University Iraqi Centre for Cancer and Medical Genetics Research

Publication Date

2015-06-30

Country of Publication

Iraq

No. of Pages

7

Main Subjects

Pharmacy, Health & Medical Sciences

Abstract EN

Human papilloma viruses (HPV) have been detected in several types of cancers.

Over the last few years, a possible correlation between HPV infection and colorectal cancer has been suggested.

The aim of this study was to investigate the association between human papilloma virus (HPV) infection and colorectal cancer.

To determine the relationship between HPV and colorectal adenocarcinoma, a retrospective study was done.

This study was carried out on 50 patients with hisopathologically confirmed primary colorectal cancer.

Two samples were collected from each patient: one sample from the tumor site and the other one from adjacent normally appearing colorectal tissues, as well as ten (20) colorectal tissues from control individuals with no cancer.

In situ hybridization (ISH) was used to detect HPV DNA (HPV 16 and 18 DNA ISH and 6/11 DNA CISH) in colorectal tissues.

HPV 16 was detected in 16(32%) of tumor samples, and in 7 (14%) of adjacent normal tissues, and in additionally two cases of apparently healthy group gave positive results for it.

HPV 18 was detected in 11 (22%) of tumor samples and in 6 (12%) of adjacent normal tissue.

HPV 6/11 was detected in 24 (48%) of tumor samples and in 7 (14%) of adjacent normal tissue.Our results suggest that colorectal HPV infection is common in patients with adenocarcinoma colorectal, albeit at a low DNA copy number, with HPV16 being the most prevalent type.

HPV infection may play a role in colorectal carcinogenesis.

American Psychological Association (APA)

Jasim, Balqis Sadun& Dawud, Dawud Salman& Ali, Sad Hasan Muhammad& al-Wakil, Nizar Abd al-Hasan. 2015. Molecular localization of human papilloma virus genotypes (16, 18, and 611) in patients with colorectal cancer by DNA-Insitu hybridization. Iraqi Journal of Cancer and Medical Genetics،Vol. 8, no. 1, pp.14-20.
https://search.emarefa.net/detail/BIM-819024

Modern Language Association (MLA)

Jasim, Balqis Sadun…[et al.]. Molecular localization of human papilloma virus genotypes (16, 18, and 611) in patients with colorectal cancer by DNA-Insitu hybridization. Iraqi Journal of Cancer and Medical Genetics Vol. 8, no. 1 (2015), pp.14-20.
https://search.emarefa.net/detail/BIM-819024

American Medical Association (AMA)

Jasim, Balqis Sadun& Dawud, Dawud Salman& Ali, Sad Hasan Muhammad& al-Wakil, Nizar Abd al-Hasan. Molecular localization of human papilloma virus genotypes (16, 18, and 611) in patients with colorectal cancer by DNA-Insitu hybridization. Iraqi Journal of Cancer and Medical Genetics. 2015. Vol. 8, no. 1, pp.14-20.
https://search.emarefa.net/detail/BIM-819024

Data Type

Journal Articles

Language

English

Notes

Record ID

BIM-819024