عزل و تشخيص فيروس جدري الأغنام باستخدام تقنية تفاعل البلمرة المتسلسل

Abstract EN

This study aimed for isolation and diagnosis of sheep pox virus by polymerase chain reaction.

26 samples were collected from clinically infected animals,9 samples were selected for inoculation in chicken embryos.

Agar gel precipitation test was used for detect the reaction between the viral isolate and specific hyper immune serum, slides were prepared from chorioallantoic membrane for histopatholgical examination.

Serum neutralization test used for detecting neutralizing antibodies for isolate.

Selected 7 samples were examined by polymerase chain reaction for detection extracted nucleic acid.

The result showed firstly reported of nodular form in Nineveh province, red pock lesions appear on chorioallantoic membrane during serial passages, a precipitation lines appears between the isolate and hyper immune serum by immunodiffusion test and intra-cytoplasmic inclusion bodies appear within infected cells, and the neutralizing antibodies showed by serum neutralization test with titer 1:64.

Finally some samples showed positive result for polymerase chain reaction while another samples gave negative result.

Thus the isolation and propagation of sheep pox virus can be done on chicken embryos and diagnose by polymerase chain reaction.