Detection of mouse spermatocyte's DNA damage in vitro and in vivo using FISH assay

Abstract EN

The present study was designed in order to detection DNA damage of spermatocytes in vitro and in vivo in white mice, with explain florescence in situ hybridization FISH assay.

The study conducting on seventy (70) male mouse were kept in animals' house in college of Veterinary Medicine in Baghdad University and fed on special pellet and drank on tap water in special bottles, divided into three groups.

1st group; Thirty mice were treated (in vivo) by intra-peritoneal injection with 0.1mg/10gm B.W.

of vincristine sulfate weekly for three weeks.

2nd group; Sperms were collected from thirty mice and treated (in vitro) weekly for three weeks with 0.01mg /ml of vincristine sulfate.

3rd group; ten mice treated with intra-peritoneal injection of 0.1ml of distilled water and consider as control group.

Results were showed evident of DNA damage in TK (11qE2)/Y gene of spermatocytes, which diagnosed by using florescence microscopy after application of FISH procedure in laboratory.

The percentage of green signals and red signals indicated the defect in DNA of spermatocytes, increase percent of each refers to increase damage in chromosomes of sperms.

In conclusion, using of vincristine chemotherapy has genotoxic effects on mouse's' sperms in vivo and in vitro.