Outer membrane protein C (ompC) gene as target for diagnosis of Salmonella spp. using polymerase chain (PCR) reaction
Other Title(s)
مورث بروتين الغشاء الخارجي (ج) هدف لتشخيص جنس السالمونيلا باستخدام تفاعل البلمرة
Joint Authors
al-Janabi, Hashim Rahim Tarish
al-Jarakh, Ala Hani
Jawad, Ala Abd al-Kazim
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 15, Issue 1 (30 Jun. 2016), pp.13-17, 5 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Abstract EN
A total of 1200 different specimens collected from human and animals sources.
600 specimens from patients suffering from diarrhea who were admitted to Al-Diwaniya Teaching Hospital for Maternity and Children.
600 specimens from gall bladder (bile) of cattle from Al-Diwaniya slaughter house.
This study was conducted during the period that extended from May 2013 to April 2014.
Salmonella spp.
were isolated and identified using bacterial culturing on selective media, biochemical, API 20E, serotyping by polyvalent and conformation by PCR.
Polymerase chain reaction (PCR) was used to detect ompC gene encoding for biosynthesis of outer membrane protein C of Salmonella genus.
The results revealed that the rate of Salmonella isolates was 0.5% (3/600) from human and 1% (6/600) from animals.
The PCR technique revealed that 9 isolates of Salmonella spp.
were contain ompC gene (DNA amplification showed one distinct band with molecular weight of 204 bp when electrophorised on agarose gel).The results of this study revealed that the PCR technique had a high specificity in detection of Salmonella spp., in comparison to culture and biochemical test, Mini API 20 E and serological tests.
The present study found no significant differences between human and animal isolates.
American Psychological Association (APA)
Jawad, Ala Abd al-Kazim& al-Jarakh, Ala Hani& al-Janabi, Hashim Rahim Tarish. 2016. Outer membrane protein C (ompC) gene as target for diagnosis of Salmonella spp. using polymerase chain (PCR) reaction. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 15, no. 1, pp.13-17.
https://search.emarefa.net/detail/BIM-819075
Modern Language Association (MLA)
Jawad, Ala Abd al-Kazim…[et al.]. Outer membrane protein C (ompC) gene as target for diagnosis of Salmonella spp. using polymerase chain (PCR) reaction. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 15, no. 1 (2016), pp.13-17.
https://search.emarefa.net/detail/BIM-819075
American Medical Association (AMA)
Jawad, Ala Abd al-Kazim& al-Jarakh, Ala Hani& al-Janabi, Hashim Rahim Tarish. Outer membrane protein C (ompC) gene as target for diagnosis of Salmonella spp. using polymerase chain (PCR) reaction. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2016. Vol. 15, no. 1, pp.13-17.
https://search.emarefa.net/detail/BIM-819075
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 17
Record ID
BIM-819075