Isolation of pseudomonas aeruginosa and molecular detection of bla-OXA gene of the bacteria from milk of mastitis cattle and from the wounds of the udder

Abstract EN

The study aimed to isolate, identify (Pseudomonas aeruginosa), test the susceptibility of P.

aeruginosa isolates against some antibiotics (class A- penicillineses and D-cloxacillin-hydrolyzing enzymes (OXA)), and detection the virulence factor (Beta lactemase -OXA gene) by PCR technique.

Twenty two isolates of P.

aeruginosa (8 from the milk and 14 swabs of wound) were obtained from 70 cattle have mastitis and wounds on udder, by using nutrient agar and MacConkey agar.

The antibiotic sensitivity test was performed by disc diffusion methods using four antibiotics (oxacillin, cefotaxime, ticarcillin, and impenem).

Among the 4 antibiotics tested, the highest resistance was found with oxacillin, cefotaxime (100%, and 60%) respectively, and the lowest resistance rate was to the ticarcillin, and impenem (39%, and 55%) respectively.

PCR were performed for all the resistant strains where the frequency of bla-OXA gene have product (618bp) to 22 strains with multidrug resistance of P.

aeruginosa infection to cattle suffering mastitis.