Isolation of pseudomonas aeruginosa and molecular detection of bla-OXA gene of the bacteria from milk of mastitis cattle and from the wounds of the udder
Other Title(s)
عزل و تشخيص جزئي لجين البيتا-لاكتميزالاوكسا لجرثومة الزوائف الزنجارية Pseudomonas aeruginosa من الأبقار المصابة بالالتهاب الضرع و جروح الضرع
Joint Authors
Muhammad, Basimah Jasim
Jawad, Muhammad Sabri
al-Hasnawi, Zaynah Fuad Salih
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 15, Issue 1 (30 Jun. 2016), pp.29-33, 5 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Abstract EN
The study aimed to isolate, identify (Pseudomonas aeruginosa), test the susceptibility of P.
aeruginosa isolates against some antibiotics (class A- penicillineses and D-cloxacillin-hydrolyzing enzymes (OXA)), and detection the virulence factor (Beta lactemase -OXA gene) by PCR technique.
Twenty two isolates of P.
aeruginosa (8 from the milk and 14 swabs of wound) were obtained from 70 cattle have mastitis and wounds on udder, by using nutrient agar and MacConkey agar.
The antibiotic sensitivity test was performed by disc diffusion methods using four antibiotics (oxacillin, cefotaxime, ticarcillin, and impenem).
Among the 4 antibiotics tested, the highest resistance was found with oxacillin, cefotaxime (100%, and 60%) respectively, and the lowest resistance rate was to the ticarcillin, and impenem (39%, and 55%) respectively.
PCR were performed for all the resistant strains where the frequency of bla-OXA gene have product (618bp) to 22 strains with multidrug resistance of P.
aeruginosa infection to cattle suffering mastitis.
American Psychological Association (APA)
al-Hasnawi, Zaynah Fuad Salih& Muhammad, Basimah Jasim& Jawad, Muhammad Sabri. 2016. Isolation of pseudomonas aeruginosa and molecular detection of bla-OXA gene of the bacteria from milk of mastitis cattle and from the wounds of the udder. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 15, no. 1, pp.29-33.
https://search.emarefa.net/detail/BIM-819081
Modern Language Association (MLA)
Muhammad, Basimah Jasim…[et al.]. Isolation of pseudomonas aeruginosa and molecular detection of bla-OXA gene of the bacteria from milk of mastitis cattle and from the wounds of the udder. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 15, no. 1 (2016), pp.29-33.
https://search.emarefa.net/detail/BIM-819081
American Medical Association (AMA)
al-Hasnawi, Zaynah Fuad Salih& Muhammad, Basimah Jasim& Jawad, Muhammad Sabri. Isolation of pseudomonas aeruginosa and molecular detection of bla-OXA gene of the bacteria from milk of mastitis cattle and from the wounds of the udder. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2016. Vol. 15, no. 1, pp.29-33.
https://search.emarefa.net/detail/BIM-819081
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 32-33
Record ID
BIM-819081