Detection of E. coli isolated from cheese by using virulence factors by PCR technique in al-Diwaniyia city

Abstract EN

The study was conduct to detection of Enterohemorrhagic Escherichia coli (EHEC) by important virulence factors genes such as hemolysin toxin genes (hlyA and hlyB) by using PCR technique from cattle milk product (cheese).

Escherichia coli is an important bacterium that can cause food borne disease, the raw milk, milk products, and undercooked ground meat products are the primary sources of outbreaks.

A total 50 chees sample was collected from local market in al-diwaniyia city the study was done in period between August and October 2014.

The PCR primers for hlyA and hlyB gene were designed by this study from NCBI-Genbank published sequence.

The PCR results was show (38) positive isolates out of 50 isolates at (76%), (23) isolates producing hlyA gene and (15) isolates producing hlyB gene whereas (9) of positive isolates were producing both hemolysin toxin genes.

In conclusion, the PCR was appeared very sensitive and highly specific assay serve as suitable molecular diagnostic tool for detection Enterohemorrhagic Escherichia coli producing hemolysin toxins.