Isolation and identification of Melissococcus plutonius from European foulbrood infected beehives and in vivo using of oxytetracycline for treatment
Other Title(s)
عزل و تشخيص Melissococcus plutonius من مرض تعفن الحضنة الأوربي في خلايا النحل و تأثير استخدام الأوكسي تترسايكلين في علاجها (في الجسم الحي)
Author
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 15, Issue 1 (30 Jun. 2016), pp.139-144, 6 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
European foulbrood (EFB) is an infectious and contagious bacterial disease of honeybee larvae caused by (Melissococcus plutonius) affects primarily the young larvae (less than 48 hrs.
old), and kill mainly the unsealed larvae at the age of 4 to 5 days.
The aim of the present study was the isolation and diagnosis of M.
plutonius that cause European foulbrood disease in beehives and used oxytetracycline in treatment of this disease (in vivo).
The infected larvae were detected by the clinical signs appears; firstly the larvae become flaccid and twisted in C- shape, the affected brood appeared yellowish, cream-colored, and later brown to almost black that can easily be removed from the cells.
Sixty nine swabs samples were collected from bees cells in Hila city.
Samples were cultured on selective, differential media, and biochemical tests.
All positive samples for M.
plutonius, were subcultures on nutrient agar, blood agar and yeast extract agar, and incubated at 35˚C for a week under anaerobic condition.
Results declare presence of 57 samples (82.6%) give positive result of M.
plutonius, while 12 (17.4%) samples were negative.
Bacteria appears as a Gram-positive lanceolatecoccus, slender rods with a tendency to form chains.
Results also showed that nine days treatment with oxytertracycline decrease the mortality rate, the disease was disappear, and the infected larvae sealed and returned to normal.
In conclusion: The study found that the EFB was decreased when oxytetracycline used as treatment.
American Psychological Association (APA)
al-Ahbabi, Hamidah Hamzah. 2016. Isolation and identification of Melissococcus plutonius from European foulbrood infected beehives and in vivo using of oxytetracycline for treatment. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 15, no. 1, pp.139-144.
https://search.emarefa.net/detail/BIM-819128
Modern Language Association (MLA)
al-Ahbabi, Hamidah Hamzah. Isolation and identification of Melissococcus plutonius from European foulbrood infected beehives and in vivo using of oxytetracycline for treatment. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 15, no. 1 (2016), pp.139-144.
https://search.emarefa.net/detail/BIM-819128
American Medical Association (AMA)
al-Ahbabi, Hamidah Hamzah. Isolation and identification of Melissococcus plutonius from European foulbrood infected beehives and in vivo using of oxytetracycline for treatment. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2016. Vol. 15, no. 1, pp.139-144.
https://search.emarefa.net/detail/BIM-819128
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 143-144
Record ID
BIM-819128