Molecular study to detection and genotyping of Entamoeba spp. in cattle and sheep

Abstract EN

The aim of the study was to diagnose the Entamoeba spp.

in cattle and sheep by using PCR method, for identified some of these species used the Nested-PCR, and identified genotypes of E.

histolytica by RT-PCR and Syber Green I dye.

Forty eight (48) fecal samples (24 cows and 24 sheep) positive with microscopic test were collected from the beginning of October 2014 until the end of March 2015, then examined with PCR for detection of Entamoeba spp., species identified by Nested –PCR, then genotypes of E.

histolytica determined by amplification of SREHP gene using RT-PCR with SYBR Green I dye and melting curve analysis.

Results were recorded infection of cows and sheep by (E.

histolytica, E.

dispar, E.

bovis) in (78.5%, 28.5%, 21.5%) ratio respectively.

Five genotypes (I, II, III, IV, V) of E.

histolytica were detected.

All the genotypes infect the cows but the sheep infected with two (II,V) genotypes only.

In conclusion: E.

histolytica has wide spectrum of genotypes, some of these genotypes unable to infection sheep.