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Rapid detection of yersinia Enterocolitica by using Real-Time PCR technique in some types of foods in al-Qadisiya province
Other Title(s)
الكشف السريع عن اليرسينيا القولونية المعوية باستخدام تقنية تفاعل البوليمر السلسلي المؤلف في بعض أنواع الغذاء في محافظة القادسية
Author
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 14, Issue 1 (30 Jun. 2015), pp.34-38, 5 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2015-06-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Abstract EN
The present study was carried out for direct and rapid detection of pathogenic Y.
enterocolitica in some types of foods such as (raw milk, local sweet cheese and minced beef meat) exist in local different markets of Al-Qadisiya province.
A total of 192 samples were collected randomly from markets of Al-Qadisiya province involved 79 raw milk samples, 58 local sweet cheese samples and 55 minced beef meat samples.
Real-Time PCR technique based SYBER Green dye were used for detection of this bacteria by amplifying invasion locus protein (ail) gene which is found especially in Y.
enterocolitica, The results revealed that positive samples for this (ail) gene were 49 samples out of 79 examined raw milk (62%), also 6 samples out of 58 samples observed in local sweet cheese (10.3%) and 49 samples out of 55 samples of minced beef meat (89%).
A total of positive samples were 104 out of 192 examined samples, samples of minced beef meat recorded highly significant contamination (p<0.05) as compared samples of raw milk which occupied second rank in contamination with pathogen and then samples of local sweet cheese which recorded less degree of contamination.
The Real-time PCR technique was very specific and efficient for detection Y.
enterocolitica in variety foods, when compared with culture method which have many problems.
American Psychological Association (APA)
Abbas, Kazim Hasan. 2015. Rapid detection of yersinia Enterocolitica by using Real-Time PCR technique in some types of foods in al-Qadisiya province. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 14, no. 1, pp.34-38.
https://search.emarefa.net/detail/BIM-819178
Modern Language Association (MLA)
Abbas, Kazim Hasan. Rapid detection of yersinia Enterocolitica by using Real-Time PCR technique in some types of foods in al-Qadisiya province. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 14, no. 1 (2015), pp.34-38.
https://search.emarefa.net/detail/BIM-819178
American Medical Association (AMA)
Abbas, Kazim Hasan. Rapid detection of yersinia Enterocolitica by using Real-Time PCR technique in some types of foods in al-Qadisiya province. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2015. Vol. 14, no. 1, pp.34-38.
https://search.emarefa.net/detail/BIM-819178
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 37-38
Record ID
BIM-819178