RAPD-PCR based genetic variation of Candida albicans of animal and human origin

Abstract EN

The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay by using OPA-18 oligonucleotide for identification and genetic relation detection among different Candida albicans strains isolated from animals and humans.

Thirty isolates were obtained from human and animals and genotyped in previous study according to 25S rDNA into genotypes A,B,C and T, identification was documented by germ tubes production, chlamydospores production on Corn-Meal-Tween 80 agar, characteristic green color on Candida-CHROMagar media (CHROMagar microbiology, France), and the final identification was confirmed by HiCandida Identification kit (HiMedia, India), RAPD-PCR fingerprinting which was carried out by using OPA-18 oligonucleotide.

Results indicate that RAPD fingerprinting obtained by OPA-18 primer was not useful for C.

albicans identification and these patterns were consistent under same genotype, but there were differences among different genotypes of C.

albicans.

Also this study indicates that same genotypic patterns were genetically identical but there was different degree of genetic relation between different genotypes.

RAPD-PCR using OPA-18 primer was a very specific and sensitive method for epidemiological study at molecular level because of easy, reliable and highly sensitive to subgenotyping of C.

albicans but it was of less value in identification of this microorganism.