Identification of Campylobacter jejuni in poultry products by Real-Time PCR in al-Muthanna province

Abstract EN

The study was undertaken due to the potential contribution of poultry products is less well known in Iraq.

In this study, Campylobacter jejuni identified by application SYBR green based Real-Time PCR technique by amplification the specific hipo gene in (96) chicken tissue samples.

The results show no significant difference in detection rates between fresh (77.8%) and frozen (83.3%) poultry tissue samples.

In freshly slaughtered poultry samples, the liver detection rate (93.75 %) was higher than skin (62.5%) and meat (75%) while in frozen poultry samples, the detection rate was higher in meat (93.75 %) than skin (87.5%) and liver (68.75%).

In conclusion the study showed high contamination rates of poultry with Campylobacter jejuni in retail markets and the direct Real-Time PCR is suitable assay for screening poultry products and identification of Campylobacter jejuni pathogen.