Detection of Cryptosporidium parvum from feces samples of human and camels by using direct polymerase chain reaction assay technique
Other Title(s)
تشخيص طفيلي Cryptosporidium parvum في عينات براز الإنسان و الجمال باستخدام تقنية فحص تفاعل سلسلة البلمرة
Joint Authors
Ahmad, Hibah Shihab
Muhammad, Nuha Qasim
Abd, Amal Hasan
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 15, Issue 2 (31 Dec. 2016), pp.59-62, 4 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
4
Main Subjects
Abstract EN
The study was designed for molecular detected of Cryptosporidium parvum from human and camel by using direct Polymerase Chain Reaction assay technique.
A total of 50 fecal samples from human and 50 samples from camel collected.
The Cryptosporidium parvum positive isolates were identified by using specific primers for heat shock protein gene that designed in this study using NCBI-Genbank data base (Genbank code: GQ259151.1) and primer3 plus program for primer design.
Results show that prevalence of infection with Cryptosporidium parvum was (24%) 12 positive out of 50 human fecal samples, whereas the prevalence of infection with Cryptosporidium parvum in camel was (14%) 7 positive out of 50 fecal samples.
The study demonstrates that the direct Polymerase Chain Reaction (PCR) assay technique is a simple, rapid and valuable tool for the detection Cryptosporidium parvum.
American Psychological Association (APA)
Ahmad, Hibah Shihab& Abd, Amal Hasan& Muhammad, Nuha Qasim. 2016. Detection of Cryptosporidium parvum from feces samples of human and camels by using direct polymerase chain reaction assay technique. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 15, no. 2, pp.59-62.
https://search.emarefa.net/detail/BIM-819319
Modern Language Association (MLA)
Ahmad, Hibah Shihab…[et al.]. Detection of Cryptosporidium parvum from feces samples of human and camels by using direct polymerase chain reaction assay technique. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 15, no. 2 (2016), pp.59-62.
https://search.emarefa.net/detail/BIM-819319
American Medical Association (AMA)
Ahmad, Hibah Shihab& Abd, Amal Hasan& Muhammad, Nuha Qasim. Detection of Cryptosporidium parvum from feces samples of human and camels by using direct polymerase chain reaction assay technique. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2016. Vol. 15, no. 2, pp.59-62.
https://search.emarefa.net/detail/BIM-819319
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 62
Record ID
BIM-819319